Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

Rui Zhang; Xun Cao; Hanxiao Ying; Zemeng Liu; Kequan Chen

doi:10.3969/j.issn.1673-1689.2018.03.006

Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

Rui Zhang, Xun Cao, Hanxiao Ying, Zemeng Liu, Kequan Chen

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

Abstract

Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

Original language	English
Pages (from-to)	262-267
Number of pages	6
Journal	Journal of Food Science and Biotechnology
Volume	37
Issue number	3
DOIs	https://doi.org/10.3969/j.issn.1673-1689.2018.03.006
State	Published - 2018

Keywords

Enzyme catalytic
Lysine
Lysine cyclodeaminase
PDMS microchip
Pipecolic acid

Access to Document

10.3969/j.issn.1673-1689.2018.03.006

Cite this

@article{b212e0cd3ff7438d80883306d76584fe,

title = "Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis",

abstract = "Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.",

keywords = "Enzyme catalytic, Lysine, Lysine cyclodeaminase, PDMS microchip, Pipecolic acid",

author = "Rui Zhang and Xun Cao and Hanxiao Ying and Zemeng Liu and Kequan Chen",

year = "2018",

doi = "10.3969/j.issn.1673-1689.2018.03.006",

language = "英语",

volume = "37",

pages = "262--267",

journal = "Journal of Food Science and Biotechnology",

issn = "1673-1689",

publisher = "Editorial office of Journal of Food Science and Biotechnology",

number = "3",

}

TY - JOUR

T1 - Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

AU - Zhang, Rui

AU - Cao, Xun

AU - Ying, Hanxiao

AU - Liu, Zemeng

AU - Chen, Kequan

PY - 2018

Y1 - 2018

N2 - Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

AB - Pipecolic acid is an important precursor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate, a PDMS microchip was designed and fabricated. Effects of microchannel size, microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were: microchannel size 200 |xm; microchannel length 124.8 cm;and liquid flow rate 100 μm/min. Under the optimized conditions, a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction, the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

KW - Enzyme catalytic

KW - Lysine

KW - Lysine cyclodeaminase

KW - PDMS microchip

KW - Pipecolic acid

UR - http://www.scopus.com/inward/record.url?scp=85048268728&partnerID=8YFLogxK

U2 - 10.3969/j.issn.1673-1689.2018.03.006

DO - 10.3969/j.issn.1673-1689.2018.03.006

M3 - 文章

AN - SCOPUS:85048268728

SN - 1673-1689

VL - 37

SP - 262

EP - 267

JO - Journal of Food Science and Biotechnology

JF - Journal of Food Science and Biotechnology

IS - 3

ER -

Application of micro reactor in pipecolic acid synthesis through enzymatic catalysis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this