Cellular effects of magnetic nanoparticles explored by atomic force microscopy

Hongli Mao; Jingchao Li; Ida Dulińska-Molak; Naoki Kawazoe; Yoshihiko Takeda; Hiroaki Mamiya; Guoping Chen

doi:10.1039/c5bm00141b

Cellular effects of magnetic nanoparticles explored by atomic force microscopy

Hongli Mao, Jingchao Li, Ida Dulińska-Molak, Naoki Kawazoe, Yoshihiko Takeda, Hiroaki Mamiya, Guoping Chen

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The investigation of subtle change of cells exposed to nanomaterials is extremely essential but also challenging for nanomaterial-based biological applications. In this study, atomic force microscopy (AFM) was employed to investigate the effects of iron-iron oxide core-shell magnetic nanoparticles on the mechanical properties of bovine articular chondrocytes (BACs). After being exposed to the nanoparticles even at a high nanoparticle-concentration (50 μg mL^-1), no obvious difference was observed by using conventional methods, including the WST-1 assay and live/dead staining. However a significant difference of Young's modulus of the cells was detected by AFM even when the concentration of nanoparticles applied in the cell culture medium was low (10 μg mL^-1). The difference of cellular Young's modulus increased with the increase of nanoparticle concentration. AFM was demonstrated to be a useful tool to identify the subtle change of cells when they were exposed to nanomaterials even at a low concentration.

Original language	English
Pages (from-to)	1284-1290
Number of pages	7
Journal	Biomaterials Science
Volume	3
Issue number	9
DOIs	https://doi.org/10.1039/c5bm00141b
State	Published - 1 Sep 2015
Externally published	Yes

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title = "Cellular effects of magnetic nanoparticles explored by atomic force microscopy",

abstract = "The investigation of subtle change of cells exposed to nanomaterials is extremely essential but also challenging for nanomaterial-based biological applications. In this study, atomic force microscopy (AFM) was employed to investigate the effects of iron-iron oxide core-shell magnetic nanoparticles on the mechanical properties of bovine articular chondrocytes (BACs). After being exposed to the nanoparticles even at a high nanoparticle-concentration (50 μg mL-1), no obvious difference was observed by using conventional methods, including the WST-1 assay and live/dead staining. However a significant difference of Young's modulus of the cells was detected by AFM even when the concentration of nanoparticles applied in the cell culture medium was low (10 μg mL-1). The difference of cellular Young's modulus increased with the increase of nanoparticle concentration. AFM was demonstrated to be a useful tool to identify the subtle change of cells when they were exposed to nanomaterials even at a low concentration.",

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AU - Mao, Hongli

AU - Li, Jingchao

AU - Dulińska-Molak, Ida

AU - Kawazoe, Naoki

AU - Takeda, Yoshihiko

AU - Mamiya, Hiroaki

AU - Chen, Guoping

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The investigation of subtle change of cells exposed to nanomaterials is extremely essential but also challenging for nanomaterial-based biological applications. In this study, atomic force microscopy (AFM) was employed to investigate the effects of iron-iron oxide core-shell magnetic nanoparticles on the mechanical properties of bovine articular chondrocytes (BACs). After being exposed to the nanoparticles even at a high nanoparticle-concentration (50 μg mL-1), no obvious difference was observed by using conventional methods, including the WST-1 assay and live/dead staining. However a significant difference of Young's modulus of the cells was detected by AFM even when the concentration of nanoparticles applied in the cell culture medium was low (10 μg mL-1). The difference of cellular Young's modulus increased with the increase of nanoparticle concentration. AFM was demonstrated to be a useful tool to identify the subtle change of cells when they were exposed to nanomaterials even at a low concentration.

AB - The investigation of subtle change of cells exposed to nanomaterials is extremely essential but also challenging for nanomaterial-based biological applications. In this study, atomic force microscopy (AFM) was employed to investigate the effects of iron-iron oxide core-shell magnetic nanoparticles on the mechanical properties of bovine articular chondrocytes (BACs). After being exposed to the nanoparticles even at a high nanoparticle-concentration (50 μg mL-1), no obvious difference was observed by using conventional methods, including the WST-1 assay and live/dead staining. However a significant difference of Young's modulus of the cells was detected by AFM even when the concentration of nanoparticles applied in the cell culture medium was low (10 μg mL-1). The difference of cellular Young's modulus increased with the increase of nanoparticle concentration. AFM was demonstrated to be a useful tool to identify the subtle change of cells when they were exposed to nanomaterials even at a low concentration.

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JO - Biomaterials Science

JF - Biomaterials Science

IS - 9

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Cellular effects of magnetic nanoparticles explored by atomic force microscopy

Abstract

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