Degradation performance of polyglutamic acid and its application of calcium supplement

Tingting Xu; Shuyue Zhan; Meihui Yi; Bo Chi; Hong Xu; Chun Mao

doi:10.1002/pat.4305

Degradation performance of polyglutamic acid and its application of calcium supplement

Tingting Xu, Shuyue Zhan, Meihui Yi, Bo Chi, Hong Xu, Chun Mao

COLLEGE OF FOOD SCIENCE AND LIGHT INDUSTRY

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15 Scopus citations

Abstract

Degradable biopolymers with functional groups play a crucial role in the biomedical field. In this case, the influences of temperature and pH values on the degradation performance of poly (γ-glutamic acid) (γ-PGA) were fully explored by gel permeation chromatography. Further, γ-PGA-Ca was prepared by using calcium chloride to react with the low molecular weight γ-PGA and characterized by Fourier transform infrared, differential scanning calorimetry test, gel permeation chromatography, atomic absorption spectrophotometric, Ca²⁺ release in vivo, and cytotoxicity experiments. Furthermore, Caco-2 cell model was constructed to study the mechanism of γ-PGA-Ca intestinal absorption. Results indicated that low pH value and high temperature are the suitable conditions for the degradation of γ-PGA. It also suggested that γ-PGA-Ca can be used as calcium supplements due to its high rate of absorption.

Original language	English
Pages (from-to)	1966-1973
Number of pages	8
Journal	Polymers for Advanced Technologies
Volume	29
Issue number	7
DOIs	https://doi.org/10.1002/pat.4305
State	Published - Jul 2018

Keywords

Caco-2 cell model
cytotoxicity
degradation
polyglutamic acid
polyglutamic acid-Ca

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title = "Degradation performance of polyglutamic acid and its application of calcium supplement",

abstract = "Degradable biopolymers with functional groups play a crucial role in the biomedical field. In this case, the influences of temperature and pH values on the degradation performance of poly (γ-glutamic acid) (γ-PGA) were fully explored by gel permeation chromatography. Further, γ-PGA-Ca was prepared by using calcium chloride to react with the low molecular weight γ-PGA and characterized by Fourier transform infrared, differential scanning calorimetry test, gel permeation chromatography, atomic absorption spectrophotometric, Ca2+ release in vivo, and cytotoxicity experiments. Furthermore, Caco-2 cell model was constructed to study the mechanism of γ-PGA-Ca intestinal absorption. Results indicated that low pH value and high temperature are the suitable conditions for the degradation of γ-PGA. It also suggested that γ-PGA-Ca can be used as calcium supplements due to its high rate of absorption.",

keywords = "Caco-2 cell model, cytotoxicity, degradation, polyglutamic acid, polyglutamic acid-Ca",

author = "Tingting Xu and Shuyue Zhan and Meihui Yi and Bo Chi and Hong Xu and Chun Mao",

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T1 - Degradation performance of polyglutamic acid and its application of calcium supplement

AU - Xu, Tingting

AU - Zhan, Shuyue

AU - Yi, Meihui

AU - Chi, Bo

AU - Xu, Hong

AU - Mao, Chun

PY - 2018/7

Y1 - 2018/7

N2 - Degradable biopolymers with functional groups play a crucial role in the biomedical field. In this case, the influences of temperature and pH values on the degradation performance of poly (γ-glutamic acid) (γ-PGA) were fully explored by gel permeation chromatography. Further, γ-PGA-Ca was prepared by using calcium chloride to react with the low molecular weight γ-PGA and characterized by Fourier transform infrared, differential scanning calorimetry test, gel permeation chromatography, atomic absorption spectrophotometric, Ca2+ release in vivo, and cytotoxicity experiments. Furthermore, Caco-2 cell model was constructed to study the mechanism of γ-PGA-Ca intestinal absorption. Results indicated that low pH value and high temperature are the suitable conditions for the degradation of γ-PGA. It also suggested that γ-PGA-Ca can be used as calcium supplements due to its high rate of absorption.

AB - Degradable biopolymers with functional groups play a crucial role in the biomedical field. In this case, the influences of temperature and pH values on the degradation performance of poly (γ-glutamic acid) (γ-PGA) were fully explored by gel permeation chromatography. Further, γ-PGA-Ca was prepared by using calcium chloride to react with the low molecular weight γ-PGA and characterized by Fourier transform infrared, differential scanning calorimetry test, gel permeation chromatography, atomic absorption spectrophotometric, Ca2+ release in vivo, and cytotoxicity experiments. Furthermore, Caco-2 cell model was constructed to study the mechanism of γ-PGA-Ca intestinal absorption. Results indicated that low pH value and high temperature are the suitable conditions for the degradation of γ-PGA. It also suggested that γ-PGA-Ca can be used as calcium supplements due to its high rate of absorption.

KW - Caco-2 cell model

KW - cytotoxicity

KW - degradation

KW - polyglutamic acid

KW - polyglutamic acid-Ca

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Degradation performance of polyglutamic acid and its application of calcium supplement

Abstract

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