Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal

Bingfeng Li; Xuejun He; Bo Fan; Jianlin Chu; Bingfang He

doi:10.1039/c7ra02367g

Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GT_BP1 with in situ product removal

Bingfeng Li, Xuejun He, Bo Fan, Jianlin Chu, Bingfang He

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A glycosyltransferase GT_BP1 from Bacillus pumilus BF1 was isolated. Efficient production of ponasterone A was achieved by the recombinant E. coli/gt_BP1 in a biphasic system with a molar yield of 92.7%. This in situ product removal provided the "driving force" for shifting the reaction equilibrium towards the synthesis of the product.

Original language	English
Pages (from-to)	23027-23029
Number of pages	3
Journal	RSC Advances
Volume	7
Issue number	37
DOIs	https://doi.org/10.1039/c7ra02367g
State	Published - 2017

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title = "Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal",

abstract = "A glycosyltransferase GTBP1 from Bacillus pumilus BF1 was isolated. Efficient production of ponasterone A was achieved by the recombinant E. coli/gtBP1 in a biphasic system with a molar yield of 92.7%. This in situ product removal provided the {"}driving force{"} for shifting the reaction equilibrium towards the synthesis of the product.",

author = "Bingfeng Li and Xuejun He and Bo Fan and Jianlin Chu and Bingfang He",

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AU - Li, Bingfeng

AU - He, Xuejun

AU - Fan, Bo

AU - Chu, Jianlin

AU - He, Bingfang

PY - 2017

Y1 - 2017

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AB - A glycosyltransferase GTBP1 from Bacillus pumilus BF1 was isolated. Efficient production of ponasterone A was achieved by the recombinant E. coli/gtBP1 in a biphasic system with a molar yield of 92.7%. This in situ product removal provided the "driving force" for shifting the reaction equilibrium towards the synthesis of the product.

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U2 - 10.1039/c7ra02367g

DO - 10.1039/c7ra02367g

M3 - 文章

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SN - 2046-2069

VL - 7

SP - 23027

EP - 23029

JO - RSC Advances

JF - RSC Advances

IS - 37

ER -

Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GT_BP1 with in situ product removal

Abstract

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