Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA)

Wei Xu; Xiaoji Xie; Dawei Li; Zhaoqi Yang; Tianhu Li; Xiaogang Liu

doi:10.1002/smll.201200263

Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA)

Wei Xu, Xiaoji Xie, Dawei Li, Zhaoqi Yang, Tianhu Li, Xiaogang Liu

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.

Original language	English
Pages (from-to)	1846-1850
Number of pages	5
Journal	Small
Volume	8
Issue number	12
DOIs	https://doi.org/10.1002/smll.201200263
State	Published - 25 Jun 2012
Externally published	Yes

Keywords

DNA
colorimetric detection
gold nanoparticles
nicking endonuclease
rolling circle amplification

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10.1002/smll.201200263

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title = "Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA)",

abstract = "A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.",

keywords = "DNA, colorimetric detection, gold nanoparticles, nicking endonuclease, rolling circle amplification",

author = "Wei Xu and Xiaoji Xie and Dawei Li and Zhaoqi Yang and Tianhu Li and Xiaogang Liu",

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AU - Xu, Wei

AU - Xie, Xiaoji

AU - Li, Dawei

AU - Yang, Zhaoqi

AU - Li, Tianhu

AU - Liu, Xiaogang

PY - 2012/6/25

Y1 - 2012/6/25

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AB - A combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA) is used for the rapid, colorimetric detection of DNA. The integration of rolling circle amplification into the NEANA approach allows for detection of oligonucleotides with arbitrary sequences at ultralow concentrations.

KW - DNA

KW - colorimetric detection

KW - gold nanoparticles

KW - nicking endonuclease

KW - rolling circle amplification

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U2 - 10.1002/smll.201200263

DO - 10.1002/smll.201200263

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SN - 1613-6810

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ER -

Ultrasensitive colorimetric DNA detection using a combination of rolling circle amplification and nicking endonuclease-assisted nanoparticle amplification (NEANA)

Abstract

Keywords

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