TY - JOUR
T1 - Patterned Photonic Nitrocellulose for Pseudopaper ELISA
AU - Chi, Junjie
AU - Gao, Bingbing
AU - Sun, Mi
AU - Zhang, Fengling
AU - Su, Enben
AU - Liu, Hong
AU - Gu, Zhongze
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/7/18
Y1 - 2017/7/18
N2 - We report an enzyme-link immunosorbent assay (ELISA) based on patterned pseudopaper that is made of photonic nitrocellulose for highly sensitive fluorescence bioanalysis. The pseudopaper is fabricated using self-assembled monodisperse SiO2 nanoparticles that are patterned on a polypropylene substrate as template. The self-assembled nanoparticles have a close-packed hexagonal (opal) structure, so the resulting nitrocellulose has a complementary (inverse opal) photonic structure. Owing to the slow-photon effect of the photonic structure, fluorescence emission for ELISA is enhanced by up to 57-fold without increasing the assay time or complexity. As the detection signal is significantly amplified, a simple smartphone camera suffices to serve as the detector for rapid and on-site analysis. As a demonstration, human IgG is quantitatively analyzed with a detection limit of 3.8 fg/mL, which is lower than that of conventional ELISA and paper-based ELISA. The consumption of sample and reagent is also reduced by 33 times compared with conventional ELISA. Therefore, the pseudopaper ELISA based on patterned photonic nitrocellulose is promising for sensitive, high-throughput bioanalysis.
AB - We report an enzyme-link immunosorbent assay (ELISA) based on patterned pseudopaper that is made of photonic nitrocellulose for highly sensitive fluorescence bioanalysis. The pseudopaper is fabricated using self-assembled monodisperse SiO2 nanoparticles that are patterned on a polypropylene substrate as template. The self-assembled nanoparticles have a close-packed hexagonal (opal) structure, so the resulting nitrocellulose has a complementary (inverse opal) photonic structure. Owing to the slow-photon effect of the photonic structure, fluorescence emission for ELISA is enhanced by up to 57-fold without increasing the assay time or complexity. As the detection signal is significantly amplified, a simple smartphone camera suffices to serve as the detector for rapid and on-site analysis. As a demonstration, human IgG is quantitatively analyzed with a detection limit of 3.8 fg/mL, which is lower than that of conventional ELISA and paper-based ELISA. The consumption of sample and reagent is also reduced by 33 times compared with conventional ELISA. Therefore, the pseudopaper ELISA based on patterned photonic nitrocellulose is promising for sensitive, high-throughput bioanalysis.
UR - http://www.scopus.com/inward/record.url?scp=85025165797&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b01732
DO - 10.1021/acs.analchem.7b01732
M3 - 文章
C2 - 28622470
AN - SCOPUS:85025165797
SN - 0003-2700
VL - 89
SP - 7727
EP - 7733
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 14
ER -