一碳资源在酵母中的利用与转化

Under the current increasingly severe climate and energy crisis, there is an urgent need to reduce dependence on fossil fuels and utilize cleaner and sustainable feedstocks such as one-carbon resources including methanol, formic acid and carbon dioxide. Yeast, as a eukaryotic organism, has great potential in the biotransformation of one-carbon compounds due to the existence of organelles, which can isolate the toxic substances produced during the metabolism of one-carbon compounds. Recently, with the in-depth understanding of methanol metabolism pathways and the gradual improvement of genetic manipulation tools, many advances have been made in producing chemicals from methanol using natural methylotrophic yeasts. Also, the development of commonly used industrial yeasts such as Saccharomyces cerevisiae and Yarrowia lipolytica as synthetic methylotrophic yeasts has also achieved remarkable achievements. Herein the research progress of yeast chassis in the transformation and utilization of one-carbon raw materials is focused. Further, the current bottlenecks and potential solutions for the biotransformation of one-carbon resources are analyzed. With the development of more precise and efficient tools and the interpretation of cellular metabolic networks, the biotransformation of one-carbon resources by yeasts will play an increasingly important role in the future of green biomanufacturing.