群体感应信号分子对Pseudomonas aeruginosa PAO1生物膜结构及产电性能的影响

As an important component of microbial fuel cells (MFCs), electroactive biofilm (EAB) is intended to reveal the response mechanism of quorum sensing (QS) signal molecules. Pseudomonas aeruginosa was inoculated in the MFC anode chamber, and two QS signaling molecules (N-butylserine lactone and quinolone) were added to observe their effects on the morphology, structure, and electrical performance of the anode biofilm. Results showed that adding 10 µmol/L N-butyl homoserine lactones (C4-HSL) or quinolone (PQS) decreased the start-up stages of the MFC in comparison to the control group by 290.33 h and 169.9 h, respectively; the highest output voltage was increased by 18.18% and 22.73% respectively; the mass transfer resistance was reduced to 9.77 Ω and 15.15 Ω respectively, and the pyocyanin content increased by 20.27% and 24.32% respectively. Scanning electron microscopy images showed that the EAB on the anode of the QS signal molecules with added MFCs, which was dominated by bacillus, was richer than that of the control group. Moreover, laser scanning confocal microscopy (CLSM) images showed that the addition of QS signal molecules significantly improved the survivability of cells in the EAB. The results suggest that the addition of QS signal molecules significantly promotes the biofilm-forming rate of electrochemically active bacteria on the anode, which strengthens its bioactivity, improves the electron transfer efficiency between EAB and electrodes, and thus enhances the power generation performance of the MFC system.