A dinuclear-copper(II) complex-based sensor for pyrophosphate and its applications to detecting pyrophosphatase activity and monitoring polymerase chain reaction

Jian Qiang; Chengcheng Chang; Zhengang Zhu; Tingwen Wei; Wenxiang Yu; Fang Wang; Jun Yin; Yong Wang; Wei Zhang; Jingjing Xie; Xiaoqiang Chen

doi:10.1016/j.snb.2016.04.082

A dinuclear-copper(II) complex-based sensor for pyrophosphate and its applications to detecting pyrophosphatase activity and monitoring polymerase chain reaction

Jian Qiang, Chengcheng Chang, Zhengang Zhu, Tingwen Wei, Wenxiang Yu, Fang Wang, Jun Yin, Yong Wang, Wei Zhang, Jingjing Xie, Xiaoqiang Chen

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A new dinuclear-copper(II) complex (Cu-L₂) with two ammonium arms based on bis-2-((pyridin-2-ylmethylamino)methyl)phenol as the coordinated unit was synthesized. The complex exhibited high affinity with pyrophosphate (PPi) in aqueous solution. Using pyrocatechol violet (PV) as a colorimetric indicator, the indicator displacement assay (IDA) was carried out to determine the binding constant between the complex and PPi. A ca. 784-fold enhancement was founded when compared Cu-L₂ to ammonium-free counterpart (Cu-L₁). The new dinuclear complex was successfully used to monitor the hydrolysis of PPi catalyzed by pyrophosphatase (PPase). Furthermore, by employing esculetine as a fluorescent indicator, the complex was also used to detect PPi released during polymerase chain reaction (PCR).

Original language	English
Pages (from-to)	591-598
Number of pages	8
Journal	Sensors and Actuators B: Chemical
Volume	233
DOIs	https://doi.org/10.1016/j.snb.2016.04.082
State	Published - 5 Oct 2016

Keywords

Ammonium arms
Dinuclear-copper(II) complex
IDA
PCR
PPase
PPi sensor

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10.1016/j.snb.2016.04.082

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title = "A dinuclear-copper(II) complex-based sensor for pyrophosphate and its applications to detecting pyrophosphatase activity and monitoring polymerase chain reaction",

abstract = "A new dinuclear-copper(II) complex (Cu-L2) with two ammonium arms based on bis-2-((pyridin-2-ylmethylamino)methyl)phenol as the coordinated unit was synthesized. The complex exhibited high affinity with pyrophosphate (PPi) in aqueous solution. Using pyrocatechol violet (PV) as a colorimetric indicator, the indicator displacement assay (IDA) was carried out to determine the binding constant between the complex and PPi. A ca. 784-fold enhancement was founded when compared Cu-L2 to ammonium-free counterpart (Cu-L1). The new dinuclear complex was successfully used to monitor the hydrolysis of PPi catalyzed by pyrophosphatase (PPase). Furthermore, by employing esculetine as a fluorescent indicator, the complex was also used to detect PPi released during polymerase chain reaction (PCR).",

keywords = "Ammonium arms, Dinuclear-copper(II) complex, IDA, PCR, PPase, PPi sensor",

author = "Jian Qiang and Chengcheng Chang and Zhengang Zhu and Tingwen Wei and Wenxiang Yu and Fang Wang and Jun Yin and Yong Wang and Wei Zhang and Jingjing Xie and Xiaoqiang Chen",

year = "2016",

month = oct,

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doi = "10.1016/j.snb.2016.04.082",

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journal = "Sensors and Actuators B: Chemical",

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T1 - A dinuclear-copper(II) complex-based sensor for pyrophosphate and its applications to detecting pyrophosphatase activity and monitoring polymerase chain reaction

AU - Qiang, Jian

AU - Chang, Chengcheng

AU - Zhu, Zhengang

AU - Wei, Tingwen

AU - Yu, Wenxiang

AU - Wang, Fang

AU - Yin, Jun

AU - Wang, Yong

AU - Zhang, Wei

AU - Xie, Jingjing

AU - Chen, Xiaoqiang

PY - 2016/10/5

Y1 - 2016/10/5

N2 - A new dinuclear-copper(II) complex (Cu-L2) with two ammonium arms based on bis-2-((pyridin-2-ylmethylamino)methyl)phenol as the coordinated unit was synthesized. The complex exhibited high affinity with pyrophosphate (PPi) in aqueous solution. Using pyrocatechol violet (PV) as a colorimetric indicator, the indicator displacement assay (IDA) was carried out to determine the binding constant between the complex and PPi. A ca. 784-fold enhancement was founded when compared Cu-L2 to ammonium-free counterpart (Cu-L1). The new dinuclear complex was successfully used to monitor the hydrolysis of PPi catalyzed by pyrophosphatase (PPase). Furthermore, by employing esculetine as a fluorescent indicator, the complex was also used to detect PPi released during polymerase chain reaction (PCR).

AB - A new dinuclear-copper(II) complex (Cu-L2) with two ammonium arms based on bis-2-((pyridin-2-ylmethylamino)methyl)phenol as the coordinated unit was synthesized. The complex exhibited high affinity with pyrophosphate (PPi) in aqueous solution. Using pyrocatechol violet (PV) as a colorimetric indicator, the indicator displacement assay (IDA) was carried out to determine the binding constant between the complex and PPi. A ca. 784-fold enhancement was founded when compared Cu-L2 to ammonium-free counterpart (Cu-L1). The new dinuclear complex was successfully used to monitor the hydrolysis of PPi catalyzed by pyrophosphatase (PPase). Furthermore, by employing esculetine as a fluorescent indicator, the complex was also used to detect PPi released during polymerase chain reaction (PCR).

KW - Ammonium arms

KW - Dinuclear-copper(II) complex

KW - IDA

KW - PCR

KW - PPase

KW - PPi sensor

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U2 - 10.1016/j.snb.2016.04.082

DO - 10.1016/j.snb.2016.04.082

M3 - 文章

AN - SCOPUS:84964577743

SN - 0925-4005

VL - 233

SP - 591

EP - 598

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

A dinuclear-copper(II) complex-based sensor for pyrophosphate and its applications to detecting pyrophosphatase activity and monitoring polymerase chain reaction

Abstract

Keywords

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