A fluorescent probe with high selectivity to glutathione over cysteine and homocysteine based on positive effect of carboxyl on nucleophilic substitution in CTAB

Li Wang, Haiyan Chen, Huilin Wang, Fang Wang, Srinivasulu Kambam, Yong Wang, Wenbo Zhao, Xiaoqiang Chen

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

A new fluorescent probe based on fluorescein fluorophore displays an excellent selectivity and sensitivity for GSH in buffer solution containing CTAB. The fluorescence intensity of sensor is linearly proportional to GSH concentrations ranging from 0 to 10 μM, and as low as 22 nM concentration of GSH was evaluated with 3.3 σ/s. The optical changes are attributed to the release of free fluorescein after the electron withdrawing dinitrophenyl ether reacted efficiently with GSH in CTAB micelles. The enhanced selectivity toward GSH over Cys and Hcy considered to be related to the carboxyl groups in thiols. The confocal microscopy experiments implied that this probe is a promising candidate for imaging of GSH in living cells.

Original languageEnglish
Pages (from-to)708-713
Number of pages6
JournalSensors and Actuators B: Chemical
Volume192
DOIs
StatePublished - 1 Mar 2014

Keywords

  • CTAB
  • Fluorescein-based derivative
  • Fluorescent sensor
  • GSH

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