A mitochondria-targeted fluorescent probe based on fluorescein derivative for detection of hypochlorite in living cells

Jing Lv; Yahui Chen; Fang Wang; Tingwen Wei; Zhijie Zhang; Jian Qiang; Xiaoqiang Chen

doi:10.1016/j.dyepig.2017.09.037

A mitochondria-targeted fluorescent probe based on fluorescein derivative for detection of hypochlorite in living cells

Jing Lv, Yahui Chen, Fang Wang, Tingwen Wei, Zhijie Zhang, Jian Qiang, Xiaoqiang Chen

College of Chemical Engineering

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50 μM, a linear correlation between the fluorescence intensity of FL at 530 nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068 μM. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL might be further used to investigate biological functions and pathological roles of HOCl at organelle levels.

Original language	English
Pages (from-to)	353-358
Number of pages	6
Journal	Dyes and Pigments
Volume	148
DOIs	https://doi.org/10.1016/j.dyepig.2017.09.037
State	Published - Jan 2018

Keywords

Fluorescein
Fluorescent probe
Hypochlorite
Mitochondria targetable

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10.1016/j.dyepig.2017.09.037

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title = "A mitochondria-targeted fluorescent probe based on fluorescein derivative for detection of hypochlorite in living cells",

abstract = "In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50 μM, a linear correlation between the fluorescence intensity of FL at 530 nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068 μM. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL might be further used to investigate biological functions and pathological roles of HOCl at organelle levels.",

keywords = "Fluorescein, Fluorescent probe, Hypochlorite, Mitochondria targetable",

author = "Jing Lv and Yahui Chen and Fang Wang and Tingwen Wei and Zhijie Zhang and Jian Qiang and Xiaoqiang Chen",

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year = "2018",

month = jan,

doi = "10.1016/j.dyepig.2017.09.037",

language = "英语",

volume = "148",

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T1 - A mitochondria-targeted fluorescent probe based on fluorescein derivative for detection of hypochlorite in living cells

AU - Lv, Jing

AU - Chen, Yahui

AU - Wang, Fang

AU - Wei, Tingwen

AU - Zhang, Zhijie

AU - Qiang, Jian

AU - Chen, Xiaoqiang

PY - 2018/1

Y1 - 2018/1

N2 - In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50 μM, a linear correlation between the fluorescence intensity of FL at 530 nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068 μM. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL might be further used to investigate biological functions and pathological roles of HOCl at organelle levels.

AB - In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50 μM, a linear correlation between the fluorescence intensity of FL at 530 nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068 μM. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL might be further used to investigate biological functions and pathological roles of HOCl at organelle levels.

KW - Fluorescein

KW - Fluorescent probe

KW - Hypochlorite

KW - Mitochondria targetable

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DO - 10.1016/j.dyepig.2017.09.037

M3 - 文章

AN - SCOPUS:85029722310

SN - 0143-7208

VL - 148

SP - 353

EP - 358

JO - Dyes and Pigments

JF - Dyes and Pigments

ER -

A mitochondria-targeted fluorescent probe based on fluorescein derivative for detection of hypochlorite in living cells

Abstract

Keywords

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