A novel coumarin-fluorescein-based fluorescent probe for ultrafast and visual detection of H2S in a Parkinson's disease model

Qian Shen; Zhongxi Huang; Duoteng Zhang; Xingwei Chen; Fangning Du; Zhiqiang Zhou; Chan Fu; Lin Li; Naidi Yang; Changmin Yu

doi:10.1016/j.saa.2023.123567

A novel coumarin-fluorescein-based fluorescent probe for ultrafast and visual detection of H₂S in a Parkinson's disease model

Qian Shen, Zhongxi Huang, Duoteng Zhang, Xingwei Chen, Fangning Du, Zhiqiang Zhou, Chan Fu, Lin Li, Naidi Yang, Changmin Yu

SCHOOL OF FLEXIBLE ELECTRONICS(FUTURE TECHNOLOGIES)|INSTITUTE OF ADVANCED MATERIALS(IAM)

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Hydrogen sulfide (H₂S) has a crucial impact on diverse biological processes and has been shown to be related to various diseases. Many probes have been developed to detect intracellular H₂S by fluorescent imaging. However, the development of rapid, highly selective and sensitive H₂S probes remains a challenge. Herein, two fluorogenic probes, CNS and FCS, are designed and synthesized for the ultrafast detection of H₂S with fluorescein and coumarin fluorophores. The results show that both probes can be applied to monitor and image endogenous H₂S in cervical cancer HeLa cells and live zebrafish, and FCS shows a higher sensitivity, selectivity and fluorescence intensity. We then further applied FCS in a Parkinson's disease Drosophila model, and the results show that FCS can precisely indicate the level of H₂S in the Parkinson's disease model. Thus, FCS will likely to be applied for the early diagnosis of Parkinson's disease.

Original language	English
Article number	123567
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	306
DOIs	https://doi.org/10.1016/j.saa.2023.123567
State	Published - 5 Feb 2024

Keywords

Bioimaging
Coumarin-fluorescein-based
Hydrogen sulfide probes
Parkinson's disease

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.saa.2023.123567

Cite this

@article{b9f9e46fb2e342d4a90a2bf24d46e6f8,

title = "A novel coumarin-fluorescein-based fluorescent probe for ultrafast and visual detection of H2S in a Parkinson's disease model",

abstract = "Hydrogen sulfide (H2S) has a crucial impact on diverse biological processes and has been shown to be related to various diseases. Many probes have been developed to detect intracellular H2S by fluorescent imaging. However, the development of rapid, highly selective and sensitive H2S probes remains a challenge. Herein, two fluorogenic probes, CNS and FCS, are designed and synthesized for the ultrafast detection of H2S with fluorescein and coumarin fluorophores. The results show that both probes can be applied to monitor and image endogenous H2S in cervical cancer HeLa cells and live zebrafish, and FCS shows a higher sensitivity, selectivity and fluorescence intensity. We then further applied FCS in a Parkinson's disease Drosophila model, and the results show that FCS can precisely indicate the level of H2S in the Parkinson's disease model. Thus, FCS will likely to be applied for the early diagnosis of Parkinson's disease.",

keywords = "Bioimaging, Coumarin-fluorescein-based, Hydrogen sulfide probes, Parkinson's disease",

author = "Qian Shen and Zhongxi Huang and Duoteng Zhang and Xingwei Chen and Fangning Du and Zhiqiang Zhou and Chan Fu and Lin Li and Naidi Yang and Changmin Yu",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2024",

month = feb,

day = "5",

doi = "10.1016/j.saa.2023.123567",

language = "英语",

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journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

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TY - JOUR

T1 - A novel coumarin-fluorescein-based fluorescent probe for ultrafast and visual detection of H2S in a Parkinson's disease model

AU - Shen, Qian

AU - Huang, Zhongxi

AU - Zhang, Duoteng

AU - Chen, Xingwei

AU - Du, Fangning

AU - Zhou, Zhiqiang

AU - Fu, Chan

AU - Li, Lin

AU - Yang, Naidi

AU - Yu, Changmin

PY - 2024/2/5

Y1 - 2024/2/5

N2 - Hydrogen sulfide (H2S) has a crucial impact on diverse biological processes and has been shown to be related to various diseases. Many probes have been developed to detect intracellular H2S by fluorescent imaging. However, the development of rapid, highly selective and sensitive H2S probes remains a challenge. Herein, two fluorogenic probes, CNS and FCS, are designed and synthesized for the ultrafast detection of H2S with fluorescein and coumarin fluorophores. The results show that both probes can be applied to monitor and image endogenous H2S in cervical cancer HeLa cells and live zebrafish, and FCS shows a higher sensitivity, selectivity and fluorescence intensity. We then further applied FCS in a Parkinson's disease Drosophila model, and the results show that FCS can precisely indicate the level of H2S in the Parkinson's disease model. Thus, FCS will likely to be applied for the early diagnosis of Parkinson's disease.

AB - Hydrogen sulfide (H2S) has a crucial impact on diverse biological processes and has been shown to be related to various diseases. Many probes have been developed to detect intracellular H2S by fluorescent imaging. However, the development of rapid, highly selective and sensitive H2S probes remains a challenge. Herein, two fluorogenic probes, CNS and FCS, are designed and synthesized for the ultrafast detection of H2S with fluorescein and coumarin fluorophores. The results show that both probes can be applied to monitor and image endogenous H2S in cervical cancer HeLa cells and live zebrafish, and FCS shows a higher sensitivity, selectivity and fluorescence intensity. We then further applied FCS in a Parkinson's disease Drosophila model, and the results show that FCS can precisely indicate the level of H2S in the Parkinson's disease model. Thus, FCS will likely to be applied for the early diagnosis of Parkinson's disease.

KW - Bioimaging

KW - Coumarin-fluorescein-based

KW - Hydrogen sulfide probes

KW - Parkinson's disease

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U2 - 10.1016/j.saa.2023.123567

DO - 10.1016/j.saa.2023.123567

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AN - SCOPUS:85174726225

SN - 1386-1425

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JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

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