A novel ECL sensor for determination of carcinoembryonic antigen using reduced graphene Oxide-BaYF5:Yb, Er upconversion nanocomposites and gold nanoparticles

Linlin Zhao, Juan Li, Yuhong Liu, Yuan Wei, Jin Zhang, Jinzha Zhang, Qing Xia, Qiang Zhang, Wenbo Zhao, Xiaoqiang Chen

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF5:Yb, Er (rGO-BaYF5:Yb, Er) nanocomposites. The rGO-BaYF5:Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV-vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF5:Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001-80 ng mL-1 with a low detection limit of 0.87 pg mL-1, good selectivity and satisfactory stability.

Original languageEnglish
Pages (from-to)484-491
Number of pages8
JournalSensors and Actuators B: Chemical
Volume232
DOIs
StatePublished - 1 Sep 2016

Keywords

  • Carcinoembryonic antigen
  • Electrochemiluminescence immunosensor
  • Er nanocomposites
  • Reduced graphene Oxide-BaYF:Yb
  • Surface assembly

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