A novel naphthofluorescein-based probe for ultrasensitive point-of-care testing of zinc(II) ions and its bioimaging in living cells and zebrafishes

Xingwei Chen, Jiajia Xu, Fengtai Suo, Changmin Yu, Duoteng Zhang, Jian Chen, Qiong Wu, Su Jing, Lin Li, Wei Huang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The combination of fluorescence method with paper-based diagnostic device is quite suitable in point-of-care testing (POCT). Herein, we designed a novel hybrid fluorescein-based probe ZN-2 and investigated its fluorescent properties thoroughly in the detection of Zn2+. In comparison with the fluorescein-based probe ZN-1, ZN-2 displayed better sensitivity, long-wavelength and faster response to Zn2+ within 20 min. Interestingly, we could achieve ultrasensitive, high-throughput and visual detection in the POCT analysis of Zn2+ by anchoring this probe ZN-2 on the paper-based device. This device with satisfied performance for Zn2+ detection was achieved in real samples including cytochylema, serum and lake water. Finally, the probe ZN-2 was further applied to visualize and monitor the level changes of Zn2+ in the living cells and zebrafishes.

Original languageEnglish
Article number117949
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume229
DOIs
StatePublished - 15 Mar 2020

Keywords

  • High-throughput
  • Imaging
  • Paper-based devices
  • Point-of-care testing
  • Zinc ions

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