A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues

Wenqi Meng; Wenwen Shi; Yongchun Chen; Hao Zhang; Jie Zhao; Zhenjiang Li; Kai Xiao

doi:10.1016/j.snb.2018.10.155

A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues

Wenqi Meng, Wenwen Shi, Yongchun Chen, Hao Zhang, Jie Zhao, Zhenjiang Li, Kai Xiao

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Hydrogen polysulfide (H₂S_n) has attracted increasing attention since H₂S_n instead of H₂S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H₂S_n detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H₂S_n. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H₂S_n in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H₂S_n in living tissues.

Original language	English
Pages (from-to)	871-877
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	281
DOIs	https://doi.org/10.1016/j.snb.2018.10.155
State	Published - 15 Feb 2019

Keywords

Fluorescent probe
Hydrogen polysulfide
Living cells
Lung tissues

Access to Document

10.1016/j.snb.2018.10.155

Cite this

@article{b50945dcd51c4be6a7e2725d01665312,

title = "A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues",

abstract = "Hydrogen polysulfide (H2Sn) has attracted increasing attention since H2Sn instead of H2S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H2Sn detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H2Sn. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H2Sn in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H2Sn in living tissues.",

keywords = "Fluorescent probe, Hydrogen polysulfide, Living cells, Lung tissues",

author = "Wenqi Meng and Wenwen Shi and Yongchun Chen and Hao Zhang and Jie Zhao and Zhenjiang Li and Kai Xiao",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2019",

month = feb,

day = "15",

doi = "10.1016/j.snb.2018.10.155",

language = "英语",

volume = "281",

pages = "871--877",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues

AU - Meng, Wenqi

AU - Shi, Wenwen

AU - Chen, Yongchun

AU - Zhang, Hao

AU - Zhao, Jie

AU - Li, Zhenjiang

AU - Xiao, Kai

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Hydrogen polysulfide (H2Sn) has attracted increasing attention since H2Sn instead of H2S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H2Sn detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H2Sn. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H2Sn in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H2Sn in living tissues.

AB - Hydrogen polysulfide (H2Sn) has attracted increasing attention since H2Sn instead of H2S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H2Sn detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H2Sn. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H2Sn in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H2Sn in living tissues.

KW - Fluorescent probe

KW - Hydrogen polysulfide

KW - Living cells

KW - Lung tissues

UR - http://www.scopus.com/inward/record.url?scp=85056602492&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2018.10.155

DO - 10.1016/j.snb.2018.10.155

M3 - 文章

AN - SCOPUS:85056602492

SN - 0925-4005

VL - 281

SP - 871

EP - 877

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

A red emitting fluorescent probe for imaging mitochondrial hydrogen polysulfide in living cells and tissues

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this