A super highly sensitive glucose biosensor based on Au nanoparticles-AgCl@polyaniline hybrid material

Wei Yan; Xiaomiao Feng; Xiaojun Chen; Wenhua Hou; Jun Jie Zhu

doi:10.1016/j.bios.2007.09.002

A super highly sensitive glucose biosensor based on Au nanoparticles-AgCl@polyaniline hybrid material

Wei Yan, Xiaomiao Feng, Xiaojun Chen, Wenhua Hou, Jun Jie Zhu

Nanjing University

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Gold nanoparticles (AuNPs) with an average diameter of 5 nm were assembled on the surface of silver chloride@polyaniline (PANI) core-shell nanocomposites (AgCl@PANI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) suggested that AuNPs were incorporated on AgCl@PANI through coordination bonds instead of electrostatic interaction. The resulting AuNPs-AgCl@PANI hybrid material exhibited good electroactivity at a neutral pH environment. An amperometric glucose biosensor was developed by adsorption of glucose oxidase (GOx) on an AuNPs-AgCl@PANI modified glassy carbon (GC) electrode. AuNPs-AgCl@PANI could provide a biocompatible surface for high enzyme loading. Due to size effect, the AuNPs in the hybrid material could act as a good catalyst for both oxidation and reduction of H₂O₂. As the measurement of glucose was based on the electrochemical detection of H₂O₂ generated by enzyme-catalyzed-oxidation of glucose, the biosensor exhibited a super highly sensitive response to the analyte with a detection limit of 4 pM. Moreover, the biosensor showed good reproducibility and operation stability. The effects of some factors, such as temperature and pH value, were also studied.

Original language	English
Pages (from-to)	925-931
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	23
Issue number	7
DOIs	https://doi.org/10.1016/j.bios.2007.09.002
State	Published - 28 Feb 2008
Externally published	Yes

Keywords

AgCl@polyaniline core-shell nanocomposite
Au nanoparticles
Differential pulse voltammetry
Glucose biosensor

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10.1016/j.bios.2007.09.002

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title = "A super highly sensitive glucose biosensor based on Au nanoparticles-AgCl@polyaniline hybrid material",

abstract = "Gold nanoparticles (AuNPs) with an average diameter of 5 nm were assembled on the surface of silver chloride@polyaniline (PANI) core-shell nanocomposites (AgCl@PANI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) suggested that AuNPs were incorporated on AgCl@PANI through coordination bonds instead of electrostatic interaction. The resulting AuNPs-AgCl@PANI hybrid material exhibited good electroactivity at a neutral pH environment. An amperometric glucose biosensor was developed by adsorption of glucose oxidase (GOx) on an AuNPs-AgCl@PANI modified glassy carbon (GC) electrode. AuNPs-AgCl@PANI could provide a biocompatible surface for high enzyme loading. Due to size effect, the AuNPs in the hybrid material could act as a good catalyst for both oxidation and reduction of H2O2. As the measurement of glucose was based on the electrochemical detection of H2O2 generated by enzyme-catalyzed-oxidation of glucose, the biosensor exhibited a super highly sensitive response to the analyte with a detection limit of 4 pM. Moreover, the biosensor showed good reproducibility and operation stability. The effects of some factors, such as temperature and pH value, were also studied.",

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author = "Wei Yan and Xiaomiao Feng and Xiaojun Chen and Wenhua Hou and Zhu, {Jun Jie}",

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AU - Feng, Xiaomiao

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AU - Zhu, Jun Jie

PY - 2008/2/28

Y1 - 2008/2/28

N2 - Gold nanoparticles (AuNPs) with an average diameter of 5 nm were assembled on the surface of silver chloride@polyaniline (PANI) core-shell nanocomposites (AgCl@PANI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) suggested that AuNPs were incorporated on AgCl@PANI through coordination bonds instead of electrostatic interaction. The resulting AuNPs-AgCl@PANI hybrid material exhibited good electroactivity at a neutral pH environment. An amperometric glucose biosensor was developed by adsorption of glucose oxidase (GOx) on an AuNPs-AgCl@PANI modified glassy carbon (GC) electrode. AuNPs-AgCl@PANI could provide a biocompatible surface for high enzyme loading. Due to size effect, the AuNPs in the hybrid material could act as a good catalyst for both oxidation and reduction of H2O2. As the measurement of glucose was based on the electrochemical detection of H2O2 generated by enzyme-catalyzed-oxidation of glucose, the biosensor exhibited a super highly sensitive response to the analyte with a detection limit of 4 pM. Moreover, the biosensor showed good reproducibility and operation stability. The effects of some factors, such as temperature and pH value, were also studied.

AB - Gold nanoparticles (AuNPs) with an average diameter of 5 nm were assembled on the surface of silver chloride@polyaniline (PANI) core-shell nanocomposites (AgCl@PANI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) suggested that AuNPs were incorporated on AgCl@PANI through coordination bonds instead of electrostatic interaction. The resulting AuNPs-AgCl@PANI hybrid material exhibited good electroactivity at a neutral pH environment. An amperometric glucose biosensor was developed by adsorption of glucose oxidase (GOx) on an AuNPs-AgCl@PANI modified glassy carbon (GC) electrode. AuNPs-AgCl@PANI could provide a biocompatible surface for high enzyme loading. Due to size effect, the AuNPs in the hybrid material could act as a good catalyst for both oxidation and reduction of H2O2. As the measurement of glucose was based on the electrochemical detection of H2O2 generated by enzyme-catalyzed-oxidation of glucose, the biosensor exhibited a super highly sensitive response to the analyte with a detection limit of 4 pM. Moreover, the biosensor showed good reproducibility and operation stability. The effects of some factors, such as temperature and pH value, were also studied.

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A super highly sensitive glucose biosensor based on Au nanoparticles-AgCl@polyaniline hybrid material

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