TY - JOUR
T1 - A superoxide anion biosensor based on direct electron transfer of superoxide dismutase on sodium alginate sol-gel film and its application to monitoring of living cells
AU - Wang, Xiuhua
AU - Han, Min
AU - Bao, Jianchun
AU - Tu, Wenwen
AU - Dai, Zhihui
PY - 2012/3/2
Y1 - 2012/3/2
N2 - The direct electron transfer of superoxide dismutase (SOD) was greatly facilitated by sodium alginate (SA) sol-gel film with the formal potential of 0.14V, which was just located between O 2 -/O 2 and O 2 -/H 2O 2. The preparation of the SOD/SA modified electrode was simple without any mediators or promoters. Based on bimolecular recognition for specific reactivity of SOD/SA toward O 2 -, the SOD modified electrode was utilized to measure O 2 - with good analytical performance, such as low applied potential (0V), high selectivity (no obvious interference), wide linear range (0.44-229.88μM) and low detection limit (0.23μM) in pH 7.0 phosphate buffer solution. Furthermore, it could be successfully exploited for the determination of O 2 - released from living cells directly adhered on the modified electrode surface. Thus, the proposed O 2 - biosensor, combining with the properties of SA sol-gel film, provided a novel approach for protein immobilization, direct electron transfer study of the immobilized protein and real-time determination of O 2 - released from living cells.
AB - The direct electron transfer of superoxide dismutase (SOD) was greatly facilitated by sodium alginate (SA) sol-gel film with the formal potential of 0.14V, which was just located between O 2 -/O 2 and O 2 -/H 2O 2. The preparation of the SOD/SA modified electrode was simple without any mediators or promoters. Based on bimolecular recognition for specific reactivity of SOD/SA toward O 2 -, the SOD modified electrode was utilized to measure O 2 - with good analytical performance, such as low applied potential (0V), high selectivity (no obvious interference), wide linear range (0.44-229.88μM) and low detection limit (0.23μM) in pH 7.0 phosphate buffer solution. Furthermore, it could be successfully exploited for the determination of O 2 - released from living cells directly adhered on the modified electrode surface. Thus, the proposed O 2 - biosensor, combining with the properties of SA sol-gel film, provided a novel approach for protein immobilization, direct electron transfer study of the immobilized protein and real-time determination of O 2 - released from living cells.
KW - Direct electron transfer
KW - Living cells
KW - Sodium alginate sol-gel film
KW - Superoxide dismutase
KW - Superoxide sensor
UR - http://www.scopus.com/inward/record.url?scp=84862813383&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2011.12.045
DO - 10.1016/j.aca.2011.12.045
M3 - 文章
C2 - 22304816
AN - SCOPUS:84862813383
SN - 0003-2670
VL - 717
SP - 61
EP - 66
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -