A turn-on fluorescent probe for real-time detection of endogenous cysteine in living cells

Guomin Zhao; Wenge Yang; Fangzhao Li; Zhenmei Deng; Yonghong Hu

doi:10.1016/j.jlumin.2020.117506

A turn-on fluorescent probe for real-time detection of endogenous cysteine in living cells

Guomin Zhao, Wenge Yang, Fangzhao Li, Zhenmei Deng, Yonghong Hu

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

As a biothiol, cysteine plays an important role in physiological and pathological processes. Hence, it is very significant to detect cysteine in vivo selectively and sensitively. In this paper, we designed a large Stokes shift (104 nm) fluorescent probe (TPC-Cys) with high selectivity, high sensitivity for the detection of cysteine. Upon the addition of cysteine, TPC-Cys shows a dramatic fluorescence enhancement around 504 nm. TPC-Cys can achieve good linear detection in the range of 0–80 μM, and the detection limit is 80 nM. Moreover, TPC-Cys has been successfully applied for real-time imaging of cysteine in living cells.

Original language	English
Article number	117506
Journal	Journal of Luminescence
Volume	226
DOIs	https://doi.org/10.1016/j.jlumin.2020.117506
State	Published - Oct 2020

Keywords

Bioimaging
Cysteine
Fluorescent probe

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10.1016/j.jlumin.2020.117506

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title = "A turn-on fluorescent probe for real-time detection of endogenous cysteine in living cells",

abstract = "As a biothiol, cysteine plays an important role in physiological and pathological processes. Hence, it is very significant to detect cysteine in vivo selectively and sensitively. In this paper, we designed a large Stokes shift (104 nm) fluorescent probe (TPC-Cys) with high selectivity, high sensitivity for the detection of cysteine. Upon the addition of cysteine, TPC-Cys shows a dramatic fluorescence enhancement around 504 nm. TPC-Cys can achieve good linear detection in the range of 0–80 μM, and the detection limit is 80 nM. Moreover, TPC-Cys has been successfully applied for real-time imaging of cysteine in living cells.",

keywords = "Bioimaging, Cysteine, Fluorescent probe",

author = "Guomin Zhao and Wenge Yang and Fangzhao Li and Zhenmei Deng and Yonghong Hu",

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year = "2020",

month = oct,

doi = "10.1016/j.jlumin.2020.117506",

language = "英语",

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T1 - A turn-on fluorescent probe for real-time detection of endogenous cysteine in living cells

AU - Zhao, Guomin

AU - Yang, Wenge

AU - Li, Fangzhao

AU - Deng, Zhenmei

AU - Hu, Yonghong

PY - 2020/10

Y1 - 2020/10

N2 - As a biothiol, cysteine plays an important role in physiological and pathological processes. Hence, it is very significant to detect cysteine in vivo selectively and sensitively. In this paper, we designed a large Stokes shift (104 nm) fluorescent probe (TPC-Cys) with high selectivity, high sensitivity for the detection of cysteine. Upon the addition of cysteine, TPC-Cys shows a dramatic fluorescence enhancement around 504 nm. TPC-Cys can achieve good linear detection in the range of 0–80 μM, and the detection limit is 80 nM. Moreover, TPC-Cys has been successfully applied for real-time imaging of cysteine in living cells.

AB - As a biothiol, cysteine plays an important role in physiological and pathological processes. Hence, it is very significant to detect cysteine in vivo selectively and sensitively. In this paper, we designed a large Stokes shift (104 nm) fluorescent probe (TPC-Cys) with high selectivity, high sensitivity for the detection of cysteine. Upon the addition of cysteine, TPC-Cys shows a dramatic fluorescence enhancement around 504 nm. TPC-Cys can achieve good linear detection in the range of 0–80 μM, and the detection limit is 80 nM. Moreover, TPC-Cys has been successfully applied for real-time imaging of cysteine in living cells.

KW - Bioimaging

KW - Cysteine

KW - Fluorescent probe

UR - http://www.scopus.com/inward/record.url?scp=85087720239&partnerID=8YFLogxK

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DO - 10.1016/j.jlumin.2020.117506

M3 - 文章

AN - SCOPUS:85087720239

SN - 0022-2313

VL - 226

JO - Journal of Luminescence

JF - Journal of Luminescence

M1 - 117506

ER -

A turn-on fluorescent probe for real-time detection of endogenous cysteine in living cells

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Keywords

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