A two-enzyme cascade system for the bio-production of spermidine from putrescine

Yi Liu; Xing Guo; Xin Wang; Kequan Chen; Pingkai Ouyang

doi:10.1016/j.mcat.2021.111439

A two-enzyme cascade system for the bio-production of spermidine from putrescine

Yi Liu, Xing Guo, Xin Wang, Kequan Chen, Pingkai Ouyang

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

In vitro multiple-enzyme cascade system is becoming a promising biomanufacturing platform for producing biochemicals. Spermidine is a natural polyamine substance that is widely used in health care products and medicine. In this study, spermidine was synthesized by S-adenosylmethionine(SAM) decarboxylase and spermidine synthase through a two-enzyme cascade system in vitro. The factors influencing the efficiency of this cascade system, including the source of SAM decarboxylase, the expression conditions, the ratio of enzyme addition, reaction temperature, reaction pH, and metal ion additives were identified to determine the optimal conditions for spermidine production. With the optimized system, 3.7 g/L spermidine was finally produced from 2.5 g/L putrescine and 15 g/L SAM with the yield of 91.1 %.

Original language	English
Article number	111439
Journal	Molecular Catalysis
Volume	504
DOIs	https://doi.org/10.1016/j.mcat.2021.111439
State	Published - Mar 2021

Keywords

Bioconversion
Escherichia coli
Putrescine
Spermidine
Two-enzyme coupled catalysis system

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10.1016/j.mcat.2021.111439

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title = "A two-enzyme cascade system for the bio-production of spermidine from putrescine",

abstract = "In vitro multiple-enzyme cascade system is becoming a promising biomanufacturing platform for producing biochemicals. Spermidine is a natural polyamine substance that is widely used in health care products and medicine. In this study, spermidine was synthesized by S-adenosylmethionine(SAM) decarboxylase and spermidine synthase through a two-enzyme cascade system in vitro. The factors influencing the efficiency of this cascade system, including the source of SAM decarboxylase, the expression conditions, the ratio of enzyme addition, reaction temperature, reaction pH, and metal ion additives were identified to determine the optimal conditions for spermidine production. With the optimized system, 3.7 g/L spermidine was finally produced from 2.5 g/L putrescine and 15 g/L SAM with the yield of 91.1 %.",

keywords = "Bioconversion, Escherichia coli, Putrescine, Spermidine, Two-enzyme coupled catalysis system",

author = "Yi Liu and Xing Guo and Xin Wang and Kequan Chen and Pingkai Ouyang",

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T1 - A two-enzyme cascade system for the bio-production of spermidine from putrescine

AU - Liu, Yi

AU - Guo, Xing

AU - Wang, Xin

AU - Chen, Kequan

AU - Ouyang, Pingkai

PY - 2021/3

Y1 - 2021/3

N2 - In vitro multiple-enzyme cascade system is becoming a promising biomanufacturing platform for producing biochemicals. Spermidine is a natural polyamine substance that is widely used in health care products and medicine. In this study, spermidine was synthesized by S-adenosylmethionine(SAM) decarboxylase and spermidine synthase through a two-enzyme cascade system in vitro. The factors influencing the efficiency of this cascade system, including the source of SAM decarboxylase, the expression conditions, the ratio of enzyme addition, reaction temperature, reaction pH, and metal ion additives were identified to determine the optimal conditions for spermidine production. With the optimized system, 3.7 g/L spermidine was finally produced from 2.5 g/L putrescine and 15 g/L SAM with the yield of 91.1 %.

AB - In vitro multiple-enzyme cascade system is becoming a promising biomanufacturing platform for producing biochemicals. Spermidine is a natural polyamine substance that is widely used in health care products and medicine. In this study, spermidine was synthesized by S-adenosylmethionine(SAM) decarboxylase and spermidine synthase through a two-enzyme cascade system in vitro. The factors influencing the efficiency of this cascade system, including the source of SAM decarboxylase, the expression conditions, the ratio of enzyme addition, reaction temperature, reaction pH, and metal ion additives were identified to determine the optimal conditions for spermidine production. With the optimized system, 3.7 g/L spermidine was finally produced from 2.5 g/L putrescine and 15 g/L SAM with the yield of 91.1 %.

KW - Bioconversion

KW - Escherichia coli

KW - Putrescine

KW - Spermidine

KW - Two-enzyme coupled catalysis system

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DO - 10.1016/j.mcat.2021.111439

M3 - 文章

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SN - 2468-8231

VL - 504

JO - Molecular Catalysis

JF - Molecular Catalysis

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ER -

A two-enzyme cascade system for the bio-production of spermidine from putrescine

Abstract

Keywords

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