Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle

Jianmin Hong; Zhihui Dai

doi:10.1016/j.snb.2009.04.032

Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle

Jianmin Hong, Zhihui Dai

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Hemoglobin (Hb) was immobilized on one-dimensional (1D) gold (Au) nanoparticles to construct a novel amperometric biosensor for hydrogen peroxide (H₂O₂) and nitrite (NO₂^-). 1D Au nanoparticles were prepared by electrodepositon in the pores of an alumina template and were characterized by XRD and TEM. The immobilized Hb retained its biological activity well and displayed a good response to the reduction of both H₂O₂ and NO₂^-. Its apparent Michaelis-Menten constants for H₂O₂ and NO₂^- were 10.8 and 35.3 μM, respectively, showing a good affinity. Under optimal conditions, the biosensors could be used for the determination of H₂O₂ ranging from 0.6 to 12.4 μM and NO₂^- ranging from 0.4 to 14.8 μM. The detection limits were 2.4 × 10^-8 and 6.5 × 10^-8 M at 3σ, respectively. 1D Au nanoparticles provided a good matrix for protein immobilization and biosensor preparation.

Original language	English
Pages (from-to)	222-226
Number of pages	5
Journal	Sensors and Actuators B: Chemical
Volume	140
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2009.04.032
State	Published - 18 Jun 2009
Externally published	Yes

Keywords

Biosensors
Hemoglobin
Hydrogen peroxide
Nitrite
One-dimensional gold nanoparticles

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10.1016/j.snb.2009.04.032

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title = "Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle",

abstract = "Hemoglobin (Hb) was immobilized on one-dimensional (1D) gold (Au) nanoparticles to construct a novel amperometric biosensor for hydrogen peroxide (H2O2) and nitrite (NO2-). 1D Au nanoparticles were prepared by electrodepositon in the pores of an alumina template and were characterized by XRD and TEM. The immobilized Hb retained its biological activity well and displayed a good response to the reduction of both H2O2 and NO2-. Its apparent Michaelis-Menten constants for H2O2 and NO2- were 10.8 and 35.3 μM, respectively, showing a good affinity. Under optimal conditions, the biosensors could be used for the determination of H2O2 ranging from 0.6 to 12.4 μM and NO2- ranging from 0.4 to 14.8 μM. The detection limits were 2.4 × 10-8 and 6.5 × 10-8 M at 3σ, respectively. 1D Au nanoparticles provided a good matrix for protein immobilization and biosensor preparation.",

keywords = "Biosensors, Hemoglobin, Hydrogen peroxide, Nitrite, One-dimensional gold nanoparticles",

author = "Jianmin Hong and Zhihui Dai",

year = "2009",

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day = "18",

doi = "10.1016/j.snb.2009.04.032",

language = "英语",

volume = "140",

pages = "222--226",

journal = "Sensors and Actuators B: Chemical",

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publisher = "Elsevier B.V.",

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T1 - Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle

AU - Hong, Jianmin

AU - Dai, Zhihui

PY - 2009/6/18

Y1 - 2009/6/18

N2 - Hemoglobin (Hb) was immobilized on one-dimensional (1D) gold (Au) nanoparticles to construct a novel amperometric biosensor for hydrogen peroxide (H2O2) and nitrite (NO2-). 1D Au nanoparticles were prepared by electrodepositon in the pores of an alumina template and were characterized by XRD and TEM. The immobilized Hb retained its biological activity well and displayed a good response to the reduction of both H2O2 and NO2-. Its apparent Michaelis-Menten constants for H2O2 and NO2- were 10.8 and 35.3 μM, respectively, showing a good affinity. Under optimal conditions, the biosensors could be used for the determination of H2O2 ranging from 0.6 to 12.4 μM and NO2- ranging from 0.4 to 14.8 μM. The detection limits were 2.4 × 10-8 and 6.5 × 10-8 M at 3σ, respectively. 1D Au nanoparticles provided a good matrix for protein immobilization and biosensor preparation.

AB - Hemoglobin (Hb) was immobilized on one-dimensional (1D) gold (Au) nanoparticles to construct a novel amperometric biosensor for hydrogen peroxide (H2O2) and nitrite (NO2-). 1D Au nanoparticles were prepared by electrodepositon in the pores of an alumina template and were characterized by XRD and TEM. The immobilized Hb retained its biological activity well and displayed a good response to the reduction of both H2O2 and NO2-. Its apparent Michaelis-Menten constants for H2O2 and NO2- were 10.8 and 35.3 μM, respectively, showing a good affinity. Under optimal conditions, the biosensors could be used for the determination of H2O2 ranging from 0.6 to 12.4 μM and NO2- ranging from 0.4 to 14.8 μM. The detection limits were 2.4 × 10-8 and 6.5 × 10-8 M at 3σ, respectively. 1D Au nanoparticles provided a good matrix for protein immobilization and biosensor preparation.

KW - Biosensors

KW - Hemoglobin

KW - Hydrogen peroxide

KW - Nitrite

KW - One-dimensional gold nanoparticles

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DO - 10.1016/j.snb.2009.04.032

M3 - 文章

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SN - 0925-4005

VL - 140

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EP - 226

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1

ER -

Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle

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Keywords

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