An Au nanocomposite based biosensor for determination of cholesterol

Lin Xu, Mengdan Zhang, Yiting Hou, Wei Huang, Cheng Yao, Qiong Wu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The present study aims to construct a biosensor to detect cholesterol by ChOx/HRP/Au/APTES/ITO. We tested the electrochemical characterization of this electrochemical sensor electrode using a cyclic voltammetry (CV) technique. Meanwhile, the electron transfer properties of the electrodes after different surface modifications were characterized by electrochemical impedance spectroscopy (EIS). This study demonstrated AuNPs as efficient electron transfer mediators for enzymes based on electrochemical cholesterol biosensors. The calibration linear relationship range of cholesterol was 0.5-450 mg dL-1 with a sensitivity of 7.5 μA mg-1 dL-1 cm-2. The excellent performances of the developed biosensor indicated that it can act as a promising candidate for the electrochemical detection of cholesterol.

Original languageEnglish
Pages (from-to)3480-3485
Number of pages6
JournalAnalytical Methods
Volume7
Issue number8
DOIs
StatePublished - 21 Apr 2015

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