Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells

Shuai Fang; Chao Wang; Jian Xiang; Liang Cheng; Xuejiao Song; Ligeng Xu; Rui Peng; Zhuang Liu

doi:10.1007/s12274-014-0497-9

Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells

Shuai Fang, Chao Wang, Jian Xiang, Liang Cheng, Xuejiao Song, Ligeng Xu, Rui Peng, Zhuang Liu

Soochow University

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Detection of circulating tumor cells (CTCs) plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nanoparticles (UCNPs) are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles (MNPs) and placing in the presence of a magnetic field. Owing to the autofluorescencefree nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.

[Figure not available: see fulltext.]

Original language	English
Pages (from-to)	1327-1336
Number of pages	10
Journal	Nano Research
Volume	7
Issue number	9
DOIs	https://doi.org/10.1007/s12274-014-0497-9
State	Published - 1 Sep 2014
Externally published	Yes

Keywords

aptamer
circulating tumar cell (CTC) detection
magnetic nanoparticles
upconversion nanoparticles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12274-014-0497-9

Cite this

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title = "Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells",

abstract = "Detection of circulating tumor cells (CTCs) plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nanoparticles (UCNPs) are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles (MNPs) and placing in the presence of a magnetic field. Owing to the autofluorescencefree nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.[Figure not available: see fulltext.]",

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doi = "10.1007/s12274-014-0497-9",

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T1 - Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells

AU - Fang, Shuai

AU - Wang, Chao

AU - Xiang, Jian

AU - Cheng, Liang

AU - Song, Xuejiao

AU - Xu, Ligeng

AU - Peng, Rui

AU - Liu, Zhuang

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Detection of circulating tumor cells (CTCs) plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nanoparticles (UCNPs) are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles (MNPs) and placing in the presence of a magnetic field. Owing to the autofluorescencefree nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.[Figure not available: see fulltext.]

AB - Detection of circulating tumor cells (CTCs) plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nanoparticles (UCNPs) are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles (MNPs) and placing in the presence of a magnetic field. Owing to the autofluorescencefree nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.[Figure not available: see fulltext.]

KW - aptamer

KW - circulating tumar cell (CTC) detection

KW - magnetic nanoparticles

KW - upconversion nanoparticles

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DO - 10.1007/s12274-014-0497-9

M3 - 文章

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JO - Nano Research

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Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells

Abstract

Keywords

UN SDGs

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