TY - JOUR
T1 - Biomimetic superoxide dismutase stabilized by photopolymerization for superoxide anions biosensing and cell monitoring
AU - Yuan, Ling
AU - Liu, Suli
AU - Tu, Wenwen
AU - Zhang, Zengsong
AU - Bao, Jianchun
AU - Dai, Zhihui
PY - 2014/5/20
Y1 - 2014/5/20
N2 - Photopolymerization strategy, as one of the immobilization methods, has attracted considerable interest because of some advantages, such as easy operation, harmlessness to the biomolecules, and long storage stability. (E)-4-(4-Formylstyryl) pyridine (formylstyrylpyridine) was prepared through Heck reaction and used as a photopolymer material to immobilize biomimetic superoxide dismutase under ultraviolet irradiation (UV) irradiation in a short time. The styrylpyridinium moiety of Formylstyrylpyridine was photoreactive and formed a dimer under UV irradiation. Mn2P2O7 multilayer sheet, a novel superoxide dismutase mimic, was synthesized. The formed photopolymer can immobilize Mn2P2O7 firmly under UV irradiation. On the basis of high catalytic activity of Mn 2P2O7 biomimetic enzyme and long-term stability of Mn2P2O7-formylstyrylpyridine film, after introducing multiwalled carbon nanotubes (MWCNTs), a novel electrochemical biosensing platform called MWCNTs/Mn2P2O 7-formylstyrylpyridine for superoxide anion (O2 •-) detection was constructed. The biosensor displayed good performance for O2•- detection and provided a reliable platform to adhere living cells directly on the modified electrode surface. Therefore, the biosensor was successfully applied to vitro determination of O2•- released from living cells, which had a promising prospect for living cells monitoring and diagnosis of reactive oxygen species-related diseases.
AB - Photopolymerization strategy, as one of the immobilization methods, has attracted considerable interest because of some advantages, such as easy operation, harmlessness to the biomolecules, and long storage stability. (E)-4-(4-Formylstyryl) pyridine (formylstyrylpyridine) was prepared through Heck reaction and used as a photopolymer material to immobilize biomimetic superoxide dismutase under ultraviolet irradiation (UV) irradiation in a short time. The styrylpyridinium moiety of Formylstyrylpyridine was photoreactive and formed a dimer under UV irradiation. Mn2P2O7 multilayer sheet, a novel superoxide dismutase mimic, was synthesized. The formed photopolymer can immobilize Mn2P2O7 firmly under UV irradiation. On the basis of high catalytic activity of Mn 2P2O7 biomimetic enzyme and long-term stability of Mn2P2O7-formylstyrylpyridine film, after introducing multiwalled carbon nanotubes (MWCNTs), a novel electrochemical biosensing platform called MWCNTs/Mn2P2O 7-formylstyrylpyridine for superoxide anion (O2 •-) detection was constructed. The biosensor displayed good performance for O2•- detection and provided a reliable platform to adhere living cells directly on the modified electrode surface. Therefore, the biosensor was successfully applied to vitro determination of O2•- released from living cells, which had a promising prospect for living cells monitoring and diagnosis of reactive oxygen species-related diseases.
UR - http://www.scopus.com/inward/record.url?scp=84901272617&partnerID=8YFLogxK
U2 - 10.1021/ac403920q
DO - 10.1021/ac403920q
M3 - 文章
C2 - 24773067
AN - SCOPUS:84901272617
SN - 0003-2700
VL - 86
SP - 4783
EP - 4790
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -