Butanol production from acid hydrolyzed corn fiber with Clostridium beijerinckii mutant

Sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and the production of butanol (4.7±0.2g/L) by Clostridium beijerinckii IB4 in P2 medium. Optimal medium components were determined using fractional factorial design. NH₄HCO₃, FeSO₄·7H₂O and CaCO₃ were demonstrated to be significant components in the production of butanol. The Box-Behnken design and a corresponding quadratic model were used to predict medium components (NH₄HCO₃ 1.96g/L, FeSO₄·7H₂O 0.26g/L and CaCO₃ 3.15g/L) and butanol yield (9.5g/L). The confirmation experiment, under the predicted optimal conditions, yielded a butanol level of 9.5±0.1g/L. This study indicates that the Box-Behnken design is an effective approach for screening the optimal medium components required for the production of butanol. It also demonstrates that SACFH, which has high levels of inhibitors such as furan and phenolic compounds, may be used as a renewable carbon source in the production of biofuels.