Cell response to single-walled carbon nanotubes in hybrid porous collagen sponges

Hongli Mao; Naoki Kawazoe; Guoping Chen

doi:10.1016/j.colsurfb.2014.12.013

Cell response to single-walled carbon nanotubes in hybrid porous collagen sponges

Hongli Mao, Naoki Kawazoe, Guoping Chen

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Three-dimensional (3D) porous collagen sponges incorporated with single-walled carbon nanotubes (SWCNTs) were prepared and used for 3D culture of bovine articular chondrocytes (BACs). The pore structures of the sponges were controlled by using ice particulates as a porogen material. The responses of cells to SWCNTs were investigated in this 3D cell culture system by evaluation of cell functions and cellular uptake of SWCNTs. The results showed that cells adhered and spatially distributed in the porous sponges. The incorporation of SWCNTs in the porous sponges promoted cell proliferation and production of sulfated glycosaminoglycans (sGAG). Confocal Raman imaging revealed that SWCNTs could be internalized by cells. The hybrid porous sponges not only provided nanostructured pore surfaces to facilitate cell proliferation and extracellular matrix (ECM) secretion but also supplied nanomaterials for cellular uptake which may be useful for biomedical applications.

Original language	English
Pages (from-to)	63-69
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	126
DOIs	https://doi.org/10.1016/j.colsurfb.2014.12.013
State	Published - 1 Feb 2015
Externally published	Yes

Keywords

Cell functions
Cellular uptake
Porous sponges
Single-walled carbon nanotubes
Topography

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10.1016/j.colsurfb.2014.12.013

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title = "Cell response to single-walled carbon nanotubes in hybrid porous collagen sponges",

abstract = "Three-dimensional (3D) porous collagen sponges incorporated with single-walled carbon nanotubes (SWCNTs) were prepared and used for 3D culture of bovine articular chondrocytes (BACs). The pore structures of the sponges were controlled by using ice particulates as a porogen material. The responses of cells to SWCNTs were investigated in this 3D cell culture system by evaluation of cell functions and cellular uptake of SWCNTs. The results showed that cells adhered and spatially distributed in the porous sponges. The incorporation of SWCNTs in the porous sponges promoted cell proliferation and production of sulfated glycosaminoglycans (sGAG). Confocal Raman imaging revealed that SWCNTs could be internalized by cells. The hybrid porous sponges not only provided nanostructured pore surfaces to facilitate cell proliferation and extracellular matrix (ECM) secretion but also supplied nanomaterials for cellular uptake which may be useful for biomedical applications.",

keywords = "Cell functions, Cellular uptake, Porous sponges, Single-walled carbon nanotubes, Topography",

author = "Hongli Mao and Naoki Kawazoe and Guoping Chen",

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AU - Mao, Hongli

AU - Kawazoe, Naoki

AU - Chen, Guoping

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Three-dimensional (3D) porous collagen sponges incorporated with single-walled carbon nanotubes (SWCNTs) were prepared and used for 3D culture of bovine articular chondrocytes (BACs). The pore structures of the sponges were controlled by using ice particulates as a porogen material. The responses of cells to SWCNTs were investigated in this 3D cell culture system by evaluation of cell functions and cellular uptake of SWCNTs. The results showed that cells adhered and spatially distributed in the porous sponges. The incorporation of SWCNTs in the porous sponges promoted cell proliferation and production of sulfated glycosaminoglycans (sGAG). Confocal Raman imaging revealed that SWCNTs could be internalized by cells. The hybrid porous sponges not only provided nanostructured pore surfaces to facilitate cell proliferation and extracellular matrix (ECM) secretion but also supplied nanomaterials for cellular uptake which may be useful for biomedical applications.

AB - Three-dimensional (3D) porous collagen sponges incorporated with single-walled carbon nanotubes (SWCNTs) were prepared and used for 3D culture of bovine articular chondrocytes (BACs). The pore structures of the sponges were controlled by using ice particulates as a porogen material. The responses of cells to SWCNTs were investigated in this 3D cell culture system by evaluation of cell functions and cellular uptake of SWCNTs. The results showed that cells adhered and spatially distributed in the porous sponges. The incorporation of SWCNTs in the porous sponges promoted cell proliferation and production of sulfated glycosaminoglycans (sGAG). Confocal Raman imaging revealed that SWCNTs could be internalized by cells. The hybrid porous sponges not only provided nanostructured pore surfaces to facilitate cell proliferation and extracellular matrix (ECM) secretion but also supplied nanomaterials for cellular uptake which may be useful for biomedical applications.

KW - Cell functions

KW - Cellular uptake

KW - Porous sponges

KW - Single-walled carbon nanotubes

KW - Topography

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JO - Colloids and Surfaces B: Biointerfaces

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ER -

Cell response to single-walled carbon nanotubes in hybrid porous collagen sponges

Abstract

Keywords

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