Converting colour to length based on the coffee-ring effect for quantitative immunoassays using a ruler as readout

Dagan Zhang; Bingbing Gao; Yangtian Chen; Hong Liu

doi:10.1039/c7lc01127j

Converting colour to length based on the coffee-ring effect for quantitative immunoassays using a ruler as readout

Dagan Zhang, Bingbing Gao, Yangtian Chen, Hong Liu

Southeast University, Nanjing

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We report a method for converting the colorimetric results of an enzyme-linked immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analytes can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip on the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains correlates with the concentration of the analyte which can be measured for quantitative analysis. The method is promising for quantitative point-of-care detection of biomarkers under resource-limited settings.

Original language	English
Pages (from-to)	271-275
Number of pages	5
Journal	Lab on a Chip
Volume	18
Issue number	2
DOIs	https://doi.org/10.1039/c7lc01127j
State	Published - 21 Jan 2018
Externally published	Yes

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10.1039/c7lc01127j

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title = "Converting colour to length based on the coffee-ring effect for quantitative immunoassays using a ruler as readout",

abstract = "We report a method for converting the colorimetric results of an enzyme-linked immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analytes can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip on the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains correlates with the concentration of the analyte which can be measured for quantitative analysis. The method is promising for quantitative point-of-care detection of biomarkers under resource-limited settings.",

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AU - Zhang, Dagan

AU - Gao, Bingbing

AU - Chen, Yangtian

AU - Liu, Hong

PY - 2018/1/21

Y1 - 2018/1/21

N2 - We report a method for converting the colorimetric results of an enzyme-linked immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analytes can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip on the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains correlates with the concentration of the analyte which can be measured for quantitative analysis. The method is promising for quantitative point-of-care detection of biomarkers under resource-limited settings.

AB - We report a method for converting the colorimetric results of an enzyme-linked immunosorbent assay (ELISA) into length based on the coffee-ring effect, so that the quantitative detection of analytes can be carried out simply using a ruler. The influence of the shape and lamination of the paper strip on the test results is studied. As a demonstration, human IgG is quantitatively analyzed. It is found that the width of the colored stains correlates with the concentration of the analyte which can be measured for quantitative analysis. The method is promising for quantitative point-of-care detection of biomarkers under resource-limited settings.

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Converting colour to length based on the coffee-ring effect for quantitative immunoassays using a ruler as readout

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