Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent

Xinran Xiang; Hongbo Suo; Chao Xu; Yi Hu

doi:10.1016/j.colsurfb.2018.02.033

Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent

Xinran Xiang, Hongbo Suo, Chao Xu, Yi Hu

School of Pharmaceutical Sciences

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Chitosan-mesoporous silica SBA-15 hybrid nanomaterials (CTS-SBA-15) were synthesized by means of carboxyl functionalized ionic liquids as the coupling agent. The as-prepared CTS-SBA-15 support was characterized by TEM, FTIR, TG and nitrogen adsorption-desorption techniques. Porcine pancreas lipase (PPL) was then bound to the hybrid nanomaterials by using the cross-linking reagent glutaraldehyde (GA). Further, the parameters like cross-linking concentration, time and ratio of supports to enzyme were optimized. The property of immobilized lipase were tested in detail by enzyme activity assays. The results indicated that the hybrid nanomaterials could form three-dimensional (3D) structure with homogeneous mesoporous structures and immobilized PPL revealed excellent enzymatic performance.

Original language	English
Pages (from-to)	262-269
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	165
DOIs	https://doi.org/10.1016/j.colsurfb.2018.02.033
State	Published - 1 May 2018

Keywords

Chitosan
Covalent cross-linking
Enzyme immobilization
Ionic liquids
Lipase
Nanomaterial

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10.1016/j.colsurfb.2018.02.033

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title = "Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent",

abstract = "Chitosan-mesoporous silica SBA-15 hybrid nanomaterials (CTS-SBA-15) were synthesized by means of carboxyl functionalized ionic liquids as the coupling agent. The as-prepared CTS-SBA-15 support was characterized by TEM, FTIR, TG and nitrogen adsorption-desorption techniques. Porcine pancreas lipase (PPL) was then bound to the hybrid nanomaterials by using the cross-linking reagent glutaraldehyde (GA). Further, the parameters like cross-linking concentration, time and ratio of supports to enzyme were optimized. The property of immobilized lipase were tested in detail by enzyme activity assays. The results indicated that the hybrid nanomaterials could form three-dimensional (3D) structure with homogeneous mesoporous structures and immobilized PPL revealed excellent enzymatic performance.",

keywords = "Chitosan, Covalent cross-linking, Enzyme immobilization, Ionic liquids, Lipase, Nanomaterial",

author = "Xinran Xiang and Hongbo Suo and Chao Xu and Yi Hu",

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doi = "10.1016/j.colsurfb.2018.02.033",

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T1 - Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent

AU - Xiang, Xinran

AU - Suo, Hongbo

AU - Xu, Chao

AU - Hu, Yi

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Chitosan-mesoporous silica SBA-15 hybrid nanomaterials (CTS-SBA-15) were synthesized by means of carboxyl functionalized ionic liquids as the coupling agent. The as-prepared CTS-SBA-15 support was characterized by TEM, FTIR, TG and nitrogen adsorption-desorption techniques. Porcine pancreas lipase (PPL) was then bound to the hybrid nanomaterials by using the cross-linking reagent glutaraldehyde (GA). Further, the parameters like cross-linking concentration, time and ratio of supports to enzyme were optimized. The property of immobilized lipase were tested in detail by enzyme activity assays. The results indicated that the hybrid nanomaterials could form three-dimensional (3D) structure with homogeneous mesoporous structures and immobilized PPL revealed excellent enzymatic performance.

AB - Chitosan-mesoporous silica SBA-15 hybrid nanomaterials (CTS-SBA-15) were synthesized by means of carboxyl functionalized ionic liquids as the coupling agent. The as-prepared CTS-SBA-15 support was characterized by TEM, FTIR, TG and nitrogen adsorption-desorption techniques. Porcine pancreas lipase (PPL) was then bound to the hybrid nanomaterials by using the cross-linking reagent glutaraldehyde (GA). Further, the parameters like cross-linking concentration, time and ratio of supports to enzyme were optimized. The property of immobilized lipase were tested in detail by enzyme activity assays. The results indicated that the hybrid nanomaterials could form three-dimensional (3D) structure with homogeneous mesoporous structures and immobilized PPL revealed excellent enzymatic performance.

KW - Chitosan

KW - Covalent cross-linking

KW - Enzyme immobilization

KW - Ionic liquids

KW - Lipase

KW - Nanomaterial

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U2 - 10.1016/j.colsurfb.2018.02.033

DO - 10.1016/j.colsurfb.2018.02.033

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AN - SCOPUS:85042885612

SN - 0927-7765

VL - 165

SP - 262

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent

Abstract

Keywords

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