TY - JOUR
T1 - Cytochromes P450 in biosensing and biosynthesis applications
T2 - Recent progress and future perspectives
AU - Mi, Li
AU - Wang, Ziteng
AU - Yang, Wenge
AU - Huang, Chaofeng
AU - Zhou, Bin
AU - Hu, Yonghong
AU - Liu, Songqin
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2023/1
Y1 - 2023/1
N2 - Cytochrome P450 (CYP450s) as a large superfamily of ubiquitous heme-containing monooxygenases are widely distributed in kingdoms of life, catalyzing versatile reactions that introduce oxygen into a vast range of molecules. To improve the activity, stability and catalytic efficiency, the methods for immobilizing CYP450s on a biosensing platform and biosynthesis of complex natural products were reported. However, these biotechnologies are hampered by the limited O2 availability. In this review, we summarize and highlight a panorama of the classical methods for biosensor, nanoreactor with confinement function and cascade reactions, bioimaging for detecting CYP450s activity and biosynthesis of complex natural and unnatural products. Then, the gas-solid-liquid interface (triphase interface) is introduced for CYP450s immobilization to overcome limited O2 availability and drive the catalytic cycle of CYP450s-H2O2 system by in situ generating H2O2 and controlling interfacial wettability, which affords a convenient way for their synthetic application.
AB - Cytochrome P450 (CYP450s) as a large superfamily of ubiquitous heme-containing monooxygenases are widely distributed in kingdoms of life, catalyzing versatile reactions that introduce oxygen into a vast range of molecules. To improve the activity, stability and catalytic efficiency, the methods for immobilizing CYP450s on a biosensing platform and biosynthesis of complex natural products were reported. However, these biotechnologies are hampered by the limited O2 availability. In this review, we summarize and highlight a panorama of the classical methods for biosensor, nanoreactor with confinement function and cascade reactions, bioimaging for detecting CYP450s activity and biosynthesis of complex natural and unnatural products. Then, the gas-solid-liquid interface (triphase interface) is introduced for CYP450s immobilization to overcome limited O2 availability and drive the catalytic cycle of CYP450s-H2O2 system by in situ generating H2O2 and controlling interfacial wettability, which affords a convenient way for their synthetic application.
KW - Biosensing and biosynthesis
KW - Cytochromes P450
KW - Oxygen
KW - Triphase interface
UR - http://www.scopus.com/inward/record.url?scp=85139874841&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2022.116791
DO - 10.1016/j.trac.2022.116791
M3 - 文献综述
AN - SCOPUS:85139874841
SN - 0165-9936
VL - 158
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 116791
ER -