TY - JOUR
T1 - Design and construction of a Bacillus amyloliquefaciens cell factory for hyaluronic acid synthesis from Jerusalem artichoke inulin
AU - Ma, Yanqin
AU - Qiu, Yibin
AU - Yu, Caiyuan
AU - Li, Sha
AU - Xu, Hong
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/4/30
Y1 - 2022/4/30
N2 - Hyaluronic acid (HA), a high-value biomacromolecule, has wide applications in medical, cosmetic and food fields. Currently, employing the safe-grade microorganisms for de novo biosynthesis of HA from renewable substrates has become a promising alternative. In this study, we established a Bacillus amyloliquefaciens strain as platform for HA production from Jerusalem artichoke inulin. Firstly, the different HA and UDP-GlcUA synthase genes were introduced into B. amyloliquefaciens to construct the HA synthesis pathway. Secondly, the byproduct polysaccharides were removed by knocking sacB and epsA-O using CRISPR/Cas9n system, resulting in a 13% increase in HA production. Finally, 2.89 g/L HA with a high molecular weight of 1.5 MDa was obtained after optimizing fermentation conditions and adding osmotic agents. This study demonstrates the engineered B. amyloliquefaciens can effectively synthesize HA with Jerusalem artichoke inulin and provides a green route for HA production.
AB - Hyaluronic acid (HA), a high-value biomacromolecule, has wide applications in medical, cosmetic and food fields. Currently, employing the safe-grade microorganisms for de novo biosynthesis of HA from renewable substrates has become a promising alternative. In this study, we established a Bacillus amyloliquefaciens strain as platform for HA production from Jerusalem artichoke inulin. Firstly, the different HA and UDP-GlcUA synthase genes were introduced into B. amyloliquefaciens to construct the HA synthesis pathway. Secondly, the byproduct polysaccharides were removed by knocking sacB and epsA-O using CRISPR/Cas9n system, resulting in a 13% increase in HA production. Finally, 2.89 g/L HA with a high molecular weight of 1.5 MDa was obtained after optimizing fermentation conditions and adding osmotic agents. This study demonstrates the engineered B. amyloliquefaciens can effectively synthesize HA with Jerusalem artichoke inulin and provides a green route for HA production.
KW - Bacillus amyloliquefaciens
KW - CRISPR/Cas9n system
KW - Hyaluronic acid
KW - Jerusalem artichoke inulin
KW - Metabolic engineering
UR - http://www.scopus.com/inward/record.url?scp=85125112943&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2022.02.100
DO - 10.1016/j.ijbiomac.2022.02.100
M3 - 文章
C2 - 35202630
AN - SCOPUS:85125112943
SN - 0141-8130
VL - 205
SP - 410
EP - 418
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
