TY - JOUR
T1 - Determination of optimal conditions for ribonucleic acid production by Candida tropicalis no. 121
AU - Ren, Huajing
AU - Chen, Xiaochun
AU - Cao, Jiaming
AU - Li, Lei
AU - Bai, Jianxin
AU - Chen, Yong
AU - Xiong, Jian
AU - Ying, Hanjie
PY - 2011/8
Y1 - 2011/8
N2 - The experiments were based on multivariate statistical concepts, and response surface methodology (RSM) was applied to optimize the fermentation medium for the production of ribonucleic acid (RNA) by Candida tropicalis no. 121. The process involved the individual adjustment and optimization of various medium components at shake flask level. The two-level Plackett-Burman (PB) design was used to screen the medium components, which significantly influenced RNA production. Among seven variables, the concentrations of molasses, ZnSO4, and H3PO4 were found to be the important factors that significantly affected RNA production (confidence levels above 95%). These factors were further optimized using a central composite design (CCD) and RSM. The optimum values for the critical components were as follows: molasses 47. 21 g/L: ZnSO4 0. 048 g/L; H3PO4 1. 19 g/L. Under optimal conditions, RNA production was 2. 56 g/L, which was in excellent agreement with the predicted value (2. 561 g/L), and led to a 2. 1-fold increase compare with that using the original medium in RNA production.
AB - The experiments were based on multivariate statistical concepts, and response surface methodology (RSM) was applied to optimize the fermentation medium for the production of ribonucleic acid (RNA) by Candida tropicalis no. 121. The process involved the individual adjustment and optimization of various medium components at shake flask level. The two-level Plackett-Burman (PB) design was used to screen the medium components, which significantly influenced RNA production. Among seven variables, the concentrations of molasses, ZnSO4, and H3PO4 were found to be the important factors that significantly affected RNA production (confidence levels above 95%). These factors were further optimized using a central composite design (CCD) and RSM. The optimum values for the critical components were as follows: molasses 47. 21 g/L: ZnSO4 0. 048 g/L; H3PO4 1. 19 g/L. Under optimal conditions, RNA production was 2. 56 g/L, which was in excellent agreement with the predicted value (2. 561 g/L), and led to a 2. 1-fold increase compare with that using the original medium in RNA production.
KW - Candida tropicalis no. 121
KW - Ferment Production
KW - Response Surface Method
KW - Ribonucleic Acid
KW - Statistical Optimization
UR - http://www.scopus.com/inward/record.url?scp=79961023251&partnerID=8YFLogxK
U2 - 10.1007/s11814-011-0013-9
DO - 10.1007/s11814-011-0013-9
M3 - 文章
AN - SCOPUS:79961023251
SN - 0256-1115
VL - 28
SP - 1721
EP - 1726
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
IS - 8
ER -