Determination of organic acids and monomeric sugars in fermentation broth of succinic acid production with lignocellulose hydrolysate

In order to determine the organic acids and monomeric sugars in fermentation broth with the corn-husk hydrolysate as carbon source, aminex HPX-87H ion exclusion column was used with refractive index detector. The mobile phase was 0.005 mol/L of sulfuric acid ( pH 2. 0 ) with flow râte of 0. 6 mL/min at 55 °C. Organic acids and monomeric sugars in samples were completely separated within 16 min. AU the correlation coefficients( r) of regression equations were above 0.999 , and the recovery ranged from 99.3% to 102.1 % . The relative Standard deviations were in the range of 0.6% -1.6% . By this method, organic acids and monomeric sugars can be easily, rapidly and effectively determined.