Abstract
Objective: To establish an RP-HPLC method for determination of vorinostat and its related substances. Methods: A Kromasil-C 18 column (250 mm×4.6 mm, 5 μm) was used and the mobile phase was 0.1% H 3PO 4 (pH 3.0 adjusted by triethanolamine)-acetonitrile (70:30) for determination of vorinostat. Gradient elution was programmed for detecting related substances. The flow rate was 1.0 mL·min -1 and the detection wavelength was 241 nm. Results: Vorinostat was completely separated from the 5 known impurities. The linear range of vorinostat was 5.3~266 μg·mL -1 (r=1) and the limit of detection was 0.3 ng (S/N=3). Conclusion: The method is simple, accurate and reliable for the quality control of vorinostat.
| Original language | English |
|---|---|
| Pages (from-to) | 2467-2470 |
| Number of pages | 4 |
| Journal | Chinese Journal of New Drugs |
| Volume | 20 |
| Issue number | 24 |
| State | Published - 30 Dec 2011 |
Keywords
- Content determination
- RP-HPLC
- Related substances
- Vorinostat