Development of a multi-stage continuous fermentation strategy for docosahexaenoic acid production by Schizochytrium sp.

Dong Sheng Guo, Xiao Jun Ji, Lu Jing Ren, Feng Wei Yin, Xiao Man Sun, He Huang, Gao Zhen

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Docosahexaenoic acid (DHA) has wide-ranging benefits for normal development of the visual and nervous systems in infants. A sustainable source of DHA production through fermentation using Schizochytrium sp. has been developed. In this paper, we present the discovery of growth-uncoupled DHA production by Schizochytrium sp. and the development of corresponding kinetic models of fed-batch fermentations, which can be used to describe and predict the cell growth and substrate utilization as well as lipid and DHA production. Based on this kinetic model, a predictive model of multi-stage continuous fermentation process was established and used to analyze, optimize and design the process parameters. Optimal predicted processes of two-stage and three-stage continuous fermentation were developed and verified in lab-scale bioreactor based on the predicted process parameters. A successful three-stage continuous fermentation was achieved, which increased the lipid, DHA content and DHA productivity by 47.6, 64.3 and 97.1%, respectively, compared with two-stage continuous fermentation.

Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalBioresource Technology
Volume269
DOIs
StatePublished - Dec 2018

Keywords

  • Continuous fermentation
  • Docosahexaenoic acid
  • Kinetic modeling
  • Schizochytrium sp.

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