Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation

Miao miao Luo; Lu jing Ren; Sheng lan Chen; Xiao jun Ji; He Huang

doi:10.1007/s12257-016-0202-9

Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation

Miao miao Luo, Lu jing Ren, Sheng lan Chen, Xiao jun Ji, He Huang

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Vitamin K₂ (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.

Original language	English
Pages (from-to)	777-786
Number of pages	10
Journal	Biotechnology and Bioprocess Engineering
Volume	21
Issue number	6
DOIs	https://doi.org/10.1007/s12257-016-0202-9
State	Published - 1 Nov 2016

Keywords

Bacillus natto
extraction
menaquinone-7
optimization
sporulation ratio

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12257-016-0202-9

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title = "Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation",

abstract = "Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.",

keywords = "Bacillus natto, extraction, menaquinone-7, optimization, sporulation ratio",

author = "Luo, {Miao miao} and Ren, {Lu jing} and Chen, {Sheng lan} and Ji, {Xiao jun} and He Huang",

note = "Publisher Copyright: {\textcopyright} 2016, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg.",

year = "2016",

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doi = "10.1007/s12257-016-0202-9",

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T1 - Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation

AU - Luo, Miao miao

AU - Ren, Lu jing

AU - Chen, Sheng lan

AU - Ji, Xiao jun

AU - Huang, He

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.

AB - Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.

KW - Bacillus natto

KW - extraction

KW - menaquinone-7

KW - optimization

KW - sporulation ratio

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DO - 10.1007/s12257-016-0202-9

M3 - 文章

AN - SCOPUS:85009799801

SN - 1226-8372

VL - 21

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JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 6

ER -

Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation

Abstract

Keywords

UN SDGs

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