Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green

Z. H. Dai; F. X. Liu; G. F. Lu; J. C. Bao

doi:10.1007/s10008-007-0378-1

Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green

Z. H. Dai, F. X. Liu, G. F. Lu, J. C. Bao

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6-420 μM NADH with the detection limit of 3.8 μM. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Journal of Solid State Electrochemistry
Volume	12
Issue number	2
DOIs	https://doi.org/10.1007/s10008-007-0378-1
State	Published - Feb 2008
Externally published	Yes

Keywords

Alcohol dehydrogenase
Detection
Electropolymerization
Ethanol
Methylene green
NADH

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10.1007/s10008-007-0378-1

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title = "Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green",

abstract = "The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6-420 μM NADH with the detection limit of 3.8 μM. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.",

keywords = "Alcohol dehydrogenase, Detection, Electropolymerization, Ethanol, Methylene green, NADH",

author = "Dai, {Z. H.} and Liu, {F. X.} and Lu, {G. F.} and Bao, {J. C.}",

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TY - JOUR

T1 - Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green

AU - Dai, Z. H.

AU - Liu, F. X.

AU - Lu, G. F.

AU - Bao, J. C.

PY - 2008/2

Y1 - 2008/2

N2 - The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6-420 μM NADH with the detection limit of 3.8 μM. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.

AB - The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6-420 μM NADH with the detection limit of 3.8 μM. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.

KW - Alcohol dehydrogenase

KW - Detection

KW - Electropolymerization

KW - Ethanol

KW - Methylene green

KW - NADH

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U2 - 10.1007/s10008-007-0378-1

DO - 10.1007/s10008-007-0378-1

M3 - 文章

AN - SCOPUS:36348977525

SN - 1432-8488

VL - 12

SP - 175

EP - 180

JO - Journal of Solid State Electrochemistry

JF - Journal of Solid State Electrochemistry

IS - 2

ER -

Electrocatalytic detection of NADH and ethanol at glassy carbon electrode modified with electropolymerized films from methylene green

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Keywords

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