Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film

Xiaojun Chen; Yuanyuan Wang; Jinjun Zhou; Wei Yan; Xinghua Li; Jun Jie Zhu

doi:10.1021/ac7021376

Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film

Xiaojun Chen, Yuanyuan Wang, Jinjun Zhou, Wei Yan, Xinghua Li, Jun Jie Zhu

School of Chemistry and Moleculai Engineering

Nanjing University

Research output: Contribution to journal › Article › peer-review

242 Scopus citations

Abstract

A novel label-free immunosensor for the detection of C-reactive protein (CRP) was developed based on a three-dimensional ordered macroporous (3DOM) gold film modified electrode by using the electrochemical impedance spectroscopy (EIS) technique. The electrode was electrochemically fabricated with an inverted opal template, making the surface area of the 3DOM gold film up to 14.4 times higher than that of a classical bare flat one, characterized by the cyclic voltammetric (CV) technique. The 3DOM gold film which was composed of interconnected gold nanoparticles not only has a good biocompatible microenvironment but also promotes the increase of conductivity and stability. The CRP immunosensor was developed by covalentiy conjugating CRP antibodies with 3-mercaptopropionic acid (MPA) on the 3DOM gold film electrode. The CRP concentration was measured through the increase of impedance values in the corresponding specific binding of CRP antigen and CRP antibody. The increased electron-transfer resistance (R_et) values were proportional to the logarithmic value of CRP concentrations in the range of 0.1 to 20 ng mL ^-1. The detection of CRP levels in three sera obtained from hospital showed acceptable accuracy.

Original language	English
Pages (from-to)	2133-2140
Number of pages	8
Journal	Analytical Chemistry
Volume	80
Issue number	6
DOIs	https://doi.org/10.1021/ac7021376
State	Published - 15 Mar 2008

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@article{253f8ddea74c48ceaed686024f2375bd,

title = "Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film",

abstract = "A novel label-free immunosensor for the detection of C-reactive protein (CRP) was developed based on a three-dimensional ordered macroporous (3DOM) gold film modified electrode by using the electrochemical impedance spectroscopy (EIS) technique. The electrode was electrochemically fabricated with an inverted opal template, making the surface area of the 3DOM gold film up to 14.4 times higher than that of a classical bare flat one, characterized by the cyclic voltammetric (CV) technique. The 3DOM gold film which was composed of interconnected gold nanoparticles not only has a good biocompatible microenvironment but also promotes the increase of conductivity and stability. The CRP immunosensor was developed by covalentiy conjugating CRP antibodies with 3-mercaptopropionic acid (MPA) on the 3DOM gold film electrode. The CRP concentration was measured through the increase of impedance values in the corresponding specific binding of CRP antigen and CRP antibody. The increased electron-transfer resistance (Ret) values were proportional to the logarithmic value of CRP concentrations in the range of 0.1 to 20 ng mL -1. The detection of CRP levels in three sera obtained from hospital showed acceptable accuracy.",

author = "Xiaojun Chen and Yuanyuan Wang and Jinjun Zhou and Wei Yan and Xinghua Li and Zhu, {Jun Jie}",

year = "2008",

month = mar,

day = "15",

doi = "10.1021/ac7021376",

language = "英语",

volume = "80",

pages = "2133--2140",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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T1 - Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film

AU - Chen, Xiaojun

AU - Wang, Yuanyuan

AU - Zhou, Jinjun

AU - Yan, Wei

AU - Li, Xinghua

AU - Zhu, Jun Jie

PY - 2008/3/15

Y1 - 2008/3/15

N2 - A novel label-free immunosensor for the detection of C-reactive protein (CRP) was developed based on a three-dimensional ordered macroporous (3DOM) gold film modified electrode by using the electrochemical impedance spectroscopy (EIS) technique. The electrode was electrochemically fabricated with an inverted opal template, making the surface area of the 3DOM gold film up to 14.4 times higher than that of a classical bare flat one, characterized by the cyclic voltammetric (CV) technique. The 3DOM gold film which was composed of interconnected gold nanoparticles not only has a good biocompatible microenvironment but also promotes the increase of conductivity and stability. The CRP immunosensor was developed by covalentiy conjugating CRP antibodies with 3-mercaptopropionic acid (MPA) on the 3DOM gold film electrode. The CRP concentration was measured through the increase of impedance values in the corresponding specific binding of CRP antigen and CRP antibody. The increased electron-transfer resistance (Ret) values were proportional to the logarithmic value of CRP concentrations in the range of 0.1 to 20 ng mL -1. The detection of CRP levels in three sera obtained from hospital showed acceptable accuracy.

AB - A novel label-free immunosensor for the detection of C-reactive protein (CRP) was developed based on a three-dimensional ordered macroporous (3DOM) gold film modified electrode by using the electrochemical impedance spectroscopy (EIS) technique. The electrode was electrochemically fabricated with an inverted opal template, making the surface area of the 3DOM gold film up to 14.4 times higher than that of a classical bare flat one, characterized by the cyclic voltammetric (CV) technique. The 3DOM gold film which was composed of interconnected gold nanoparticles not only has a good biocompatible microenvironment but also promotes the increase of conductivity and stability. The CRP immunosensor was developed by covalentiy conjugating CRP antibodies with 3-mercaptopropionic acid (MPA) on the 3DOM gold film electrode. The CRP concentration was measured through the increase of impedance values in the corresponding specific binding of CRP antigen and CRP antibody. The increased electron-transfer resistance (Ret) values were proportional to the logarithmic value of CRP concentrations in the range of 0.1 to 20 ng mL -1. The detection of CRP levels in three sera obtained from hospital showed acceptable accuracy.

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U2 - 10.1021/ac7021376

DO - 10.1021/ac7021376

M3 - 文章

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AN - SCOPUS:41449091313

SN - 0003-2700

VL - 80

SP - 2133

EP - 2140

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 6

ER -

Electrochemical impedance immunosensor based on three-dimensionally ordered macroporous gold film

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