Electrochemical sensor based on a silver nanowires modified electrode for the determination of cholesterol

Lin Xu; Yiting Hou; Mengdan Zhang; Tao Cheng; Wei Huang; Cheng Yao; Qiong Wu

doi:10.1039/c5ay01164g

Electrochemical sensor based on a silver nanowires modified electrode for the determination of cholesterol

Lin Xu, Yiting Hou, Mengdan Zhang, Tao Cheng, Wei Huang, Cheng Yao, Qiong Wu

School of Chemistry and Moleculai Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

In this study, the fabrication of an amperometric cholesterol biosensor based on silver nanowires (AgNWs) and cholesterol oxidase (ChOx)-graphene oxide (GO)-chitosan (CS) film is reported. The electrochemical behaviour of the ChOx/Ag/GO/CS/ITO biosensor was studied using cyclic voltammetry (CV), which revealed that the developed biosensor possessed high sensitivity (13.628 μA mM^-1 cm^-2) and a low detection limit (0.427 mg dL^-1). The apparent Michaelis-Menten constant, KappM, of this biosensor was very low (2.813 mM), originating from the effective immobilization process and the nanowires structure of the substrate. The biosensor expressed a wide linear range up to 400 mg dL^-1 in physiological conditions (pH 7.0), making it very promising for the clinical determination of cholesterol.

Original language	English
Pages (from-to)	5649-5653
Number of pages	5
Journal	Analytical Methods
Volume	7
Issue number	13
DOIs	https://doi.org/10.1039/c5ay01164g
State	Published - 7 Jul 2015

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title = "Electrochemical sensor based on a silver nanowires modified electrode for the determination of cholesterol",

abstract = "In this study, the fabrication of an amperometric cholesterol biosensor based on silver nanowires (AgNWs) and cholesterol oxidase (ChOx)-graphene oxide (GO)-chitosan (CS) film is reported. The electrochemical behaviour of the ChOx/Ag/GO/CS/ITO biosensor was studied using cyclic voltammetry (CV), which revealed that the developed biosensor possessed high sensitivity (13.628 μA mM-1 cm-2) and a low detection limit (0.427 mg dL-1). The apparent Michaelis-Menten constant, KappM, of this biosensor was very low (2.813 mM), originating from the effective immobilization process and the nanowires structure of the substrate. The biosensor expressed a wide linear range up to 400 mg dL-1 in physiological conditions (pH 7.0), making it very promising for the clinical determination of cholesterol.",

author = "Lin Xu and Yiting Hou and Mengdan Zhang and Tao Cheng and Wei Huang and Cheng Yao and Qiong Wu",

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T1 - Electrochemical sensor based on a silver nanowires modified electrode for the determination of cholesterol

AU - Xu, Lin

AU - Hou, Yiting

AU - Zhang, Mengdan

AU - Cheng, Tao

AU - Huang, Wei

AU - Yao, Cheng

AU - Wu, Qiong

PY - 2015/7/7

Y1 - 2015/7/7

N2 - In this study, the fabrication of an amperometric cholesterol biosensor based on silver nanowires (AgNWs) and cholesterol oxidase (ChOx)-graphene oxide (GO)-chitosan (CS) film is reported. The electrochemical behaviour of the ChOx/Ag/GO/CS/ITO biosensor was studied using cyclic voltammetry (CV), which revealed that the developed biosensor possessed high sensitivity (13.628 μA mM-1 cm-2) and a low detection limit (0.427 mg dL-1). The apparent Michaelis-Menten constant, KappM, of this biosensor was very low (2.813 mM), originating from the effective immobilization process and the nanowires structure of the substrate. The biosensor expressed a wide linear range up to 400 mg dL-1 in physiological conditions (pH 7.0), making it very promising for the clinical determination of cholesterol.

AB - In this study, the fabrication of an amperometric cholesterol biosensor based on silver nanowires (AgNWs) and cholesterol oxidase (ChOx)-graphene oxide (GO)-chitosan (CS) film is reported. The electrochemical behaviour of the ChOx/Ag/GO/CS/ITO biosensor was studied using cyclic voltammetry (CV), which revealed that the developed biosensor possessed high sensitivity (13.628 μA mM-1 cm-2) and a low detection limit (0.427 mg dL-1). The apparent Michaelis-Menten constant, KappM, of this biosensor was very low (2.813 mM), originating from the effective immobilization process and the nanowires structure of the substrate. The biosensor expressed a wide linear range up to 400 mg dL-1 in physiological conditions (pH 7.0), making it very promising for the clinical determination of cholesterol.

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EP - 5653

JO - Analytical Methods

JF - Analytical Methods

IS - 13

ER -

Electrochemical sensor based on a silver nanowires modified electrode for the determination of cholesterol

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