TY - JOUR
T1 - Electrochemiluminescence aptasensor for Siglec-5 detection based on MoS2@Au nanocomposites emitter and exonuclease III-powered DNA walker
AU - Fan, Zhenqiang
AU - Yao, Bo
AU - Ding, Yuedi
AU - Xie, Minhao
AU - Zhao, Jianfeng
AU - Zhang, Kai
AU - Huang, Wei
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Lectins are highly specific binding proteins for glycoproteins which widely exist in living organisms, playing a vital role in exploring the biological evolution process, such as cellular proliferation, differentiation, carcinogenesis and apoptosis. Therefore, the content monitoring of lectin becomes particularly significant and urgent in the bioanalytical application. In this work, we fabricated an aptasensor, majorly capitalizing the eminent affinity between sialic acid-binding immunoglobulin (Ig)-like lectin 5 (Siglec-5) and nucleic acids aptamer (K19), with nontoxic MoS2@Au nanocomposites as electrochemiluminescence (ECL) emitters based on exonuclease III (Exo III)-powered DNA walker for the bioassays of Siglec-5. The DNA track was constructed on the emitters’ surface, providing a reliable platform for the DNA walker's autonomous move. In the assay, the primer DNA in the DNA duplex was replaced by Siglec-5 due to the aptamer interactions and repeatedly released to participate in the movement of the DNA walker, further triggering cascade signal amplification. Finally, our aptasensor indicates significant potential for assays of Siglec-5 with a detection limit of 8.9 pM.
AB - Lectins are highly specific binding proteins for glycoproteins which widely exist in living organisms, playing a vital role in exploring the biological evolution process, such as cellular proliferation, differentiation, carcinogenesis and apoptosis. Therefore, the content monitoring of lectin becomes particularly significant and urgent in the bioanalytical application. In this work, we fabricated an aptasensor, majorly capitalizing the eminent affinity between sialic acid-binding immunoglobulin (Ig)-like lectin 5 (Siglec-5) and nucleic acids aptamer (K19), with nontoxic MoS2@Au nanocomposites as electrochemiluminescence (ECL) emitters based on exonuclease III (Exo III)-powered DNA walker for the bioassays of Siglec-5. The DNA track was constructed on the emitters’ surface, providing a reliable platform for the DNA walker's autonomous move. In the assay, the primer DNA in the DNA duplex was replaced by Siglec-5 due to the aptamer interactions and repeatedly released to participate in the movement of the DNA walker, further triggering cascade signal amplification. Finally, our aptasensor indicates significant potential for assays of Siglec-5 with a detection limit of 8.9 pM.
KW - Aptasensor
KW - DNA walker
KW - Electrochemiluminescence
KW - MoS QDs
KW - Siglec-5
KW - Spike glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=85101362458&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2021.129592
DO - 10.1016/j.snb.2021.129592
M3 - 文章
AN - SCOPUS:85101362458
SN - 0925-4005
VL - 334
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 129592
ER -
