Abstract
A chiral HPLC method was established for the separation and determination of entecavir opticalisomers. Chiral separation of 5 entecavir opticalisomers was achieved under normal-phase chromatographic mode by the Chiralpak AD-H chiral stationary phase. The optimized chromatographic conditions were a mobile phase consisting of n-hexane-isopropanol-alcohol-diethylamine (70 : 6 : 24 : 0. 1) with a flow rate of 0. 5 mL/min, and an UV detector at 254 nm. The column temperature was set at 30 °C. The influences of the kinds and proportion of organic solvents, acid and base addictives, flow rate and column temperature on the enantio-separation were studied. Under the chromatographic conditions, 5 entecavir opticalisomers can be completely separated from each other and the resolution values were not less than 1. 9. The validated method is suitable for the quality control of entecavir.
| Original language | English |
|---|---|
| Pages (from-to) | 51-54 |
| Number of pages | 4 |
| Journal | Journal of China Pharmaceutical University |
| Volume | 43 |
| Issue number | 1 |
| State | Published - Feb 2012 |
Keywords
- Chiral separation
- Chiralpak AD-H
- Entecavir
- High performance liquid chromatography