Enantioselective separation of dansyl-dl-amino acids and some racemates on “click” functionalized native α-cyclodextrin based sub-2 μm columns

Feng Ai, Yong Wang, Chen Hui, Yanhui Yang, Timothy Thatt Yang Tan, Siu Choon Ng

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The current work demonstrates that native α-cyclodextrin, anchored onto sub-2 μm silica particles via “click” reactions and packed into a 5 cm column, was found to be effective for the resolution of 11 pairs of dansyl-dl-amino acids (DAAs) using ultra-high performance liquid chromatography (UHPLC). All DAAs were completely or partially separated on the column and the resolution achieved for 7 pairs of DAAs was significantly greater than 1.5. It was found that the buffer type exerted a profound impact on the separation. The effects of analyte substituents adjacent to the chiral center of analytes as well as operation conditions with respect to the separation efficiency were discussed. Five racemic compounds with single or double rings also got resolved on this short α-CD column to some extent.

Original languageEnglish
Pages (from-to)2289-2294
Number of pages6
JournalThe Analyst
Volume138
Issue number8
DOIs
StatePublished - 18 Mar 2013
Externally publishedYes

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