TY - JOUR
T1 - Engineering Yarrowia lipolytica to produce nutritional fatty acids
T2 - Current status and future perspectives
AU - Cao, Lizhen
AU - Yin, Mingxue
AU - Shi, Tian Qiong
AU - Lin, Lu
AU - Ledesma-Amaro, Rodrigo
AU - Ji, Xiao Jun
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/12
Y1 - 2022/12
N2 - Due to their vital physiological functions, nutritional fatty acids have great potential as nutraceutical food supplements for preventing an array of diseases such as inflammation, depression, arthritis, osteoporosis, diabetes and cancer. Microbial biosynthesis of fatty acids follows the trend of sustainable development, as it enables green, environmentally friendly and efficient production. As a natural oleaginous yeast, Yarrowia lipolytica is especially well-suited for the production of fatty acids. Moreover, it has a variety of genetic engineering tools and novel metabolic engineering strategies that make it a robust workhorse for the production of an array of value-added products. In this review, we summarize recent advances in metabolic engineering strategies for accumulating nutritional fatty acids in Y. lipolytica, including conjugated fatty acids and polyunsaturated fatty acids. In addition, the future prospects of nutritional fatty acid production using the Y. lipolytica platform are discussed in light of the current progress, challenges, and trends in this field. Finally, guidelines for future studies are also emphasized.
AB - Due to their vital physiological functions, nutritional fatty acids have great potential as nutraceutical food supplements for preventing an array of diseases such as inflammation, depression, arthritis, osteoporosis, diabetes and cancer. Microbial biosynthesis of fatty acids follows the trend of sustainable development, as it enables green, environmentally friendly and efficient production. As a natural oleaginous yeast, Yarrowia lipolytica is especially well-suited for the production of fatty acids. Moreover, it has a variety of genetic engineering tools and novel metabolic engineering strategies that make it a robust workhorse for the production of an array of value-added products. In this review, we summarize recent advances in metabolic engineering strategies for accumulating nutritional fatty acids in Y. lipolytica, including conjugated fatty acids and polyunsaturated fatty acids. In addition, the future prospects of nutritional fatty acid production using the Y. lipolytica platform are discussed in light of the current progress, challenges, and trends in this field. Finally, guidelines for future studies are also emphasized.
KW - Conjugated fatty acids
KW - Metabolic engineering
KW - Nutritional fatty acids
KW - Polyunsaturated fatty acids
KW - Yarrowia lipolytica
UR - http://www.scopus.com/inward/record.url?scp=85132924686&partnerID=8YFLogxK
U2 - 10.1016/j.synbio.2022.06.002
DO - 10.1016/j.synbio.2022.06.002
M3 - 文献综述
AN - SCOPUS:85132924686
SN - 2405-805X
VL - 7
SP - 1024
EP - 1033
JO - Synthetic and Systems Biotechnology
JF - Synthetic and Systems Biotechnology
IS - 4
ER -
