Enhancement of n-butanol production by in situ butanol removal using permeating-heating-gas stripping in acetone-butanol-ethanol fermentation

Yong Chen, Hengfei Ren, Dong Liu, Ting Zhao, Xinchi Shi, Hao Cheng, Nan Zhao, Zhenjian Li, Bingbing Li, Huanqing Niu, Wei Zhuang, Jingjing Xie, Xiaochun Chen, Jinglan Wu, Hanjie Ying

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Butanol recovery from acetone-butanol-ethanol (ABE) fed-batch fermentation using permeating-heating-gas was determined in this study. Fermentation was performed with Clostridium acetobutylicum B3 in a fibrous bed bioreactor and permeating-heating-gas stripping was used to eliminate substrate and product inhibition, which normally restrict ABE production and sugar utilization to below 20. g/L and 60. g/L, respectively. In batch fermentation (without permeating-heating-gas stripping), C. acetobutylicum B3 utilized 60. g/L glucose and produced 19.9. g/L ABE and 12. g/L butanol, while in the integrated process 290. g/L glucose was utilized and 106.27. g/L ABE and 66.09. g/L butanol were produced. The intermittent gas stripping process generated a highly concentrated condensate containing approximately 15% (w/v) butanol, 4% (w/v) acetone, a small amount of ethanol (<1%), and almost no acids, resulting in a highly concentrated butanol solution [~70% (w/v)] after phase separation. Butanol removal by permeating-heating-gas stripping has potential for commercial ABE production.

Original languageEnglish
Pages (from-to)276-284
Number of pages9
JournalBioresource Technology
Volume164
DOIs
StatePublished - Jul 2014

Keywords

  • ABE fermentation
  • Cell immobilization
  • Fed-batch fermentation
  • Permeating-heating-gas stripping

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