Abstract
The cellular location of target gene argE expressed in recombinant BL21(DE3)-pET22b-argE was determined. The effects of Zn2+ effect feeding time and concentration on the biomass production and gene expression product activity were studied. The mechanism of Zn2+ effect was analyzed. The results showed that N-acetylornithine deacetylase coded by argE gene was over expressed and most of it was expressed as insoluble inclusion body, only a little as soluble expression. 1.0 g/L Mg2+ concentration improved both the biomass production and enzyme activity greatly. With different concentrations and feeding times, Zn2+ could bring about different influences. In contrast to the inhibition of feeding Zn2+at the cultivation beginning, addition of 1.0 mg/L Zn2+ after inducing for 2.5 h with 1.0% lactose reduced the inhibition on the growth and increased the enzyme activity. SDS-PAGE analysis and enzyme activity measurement proved that Zn2+ did not influence the expression rate, but the catalyst site of recombinant NAOase.
| Original language | English |
|---|---|
| Pages (from-to) | 1187-1191 |
| Number of pages | 5 |
| Journal | Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering |
| Volume | 7 |
| Issue number | 6 |
| State | Published - Dec 2007 |
Keywords
- Activity expression
- Expression optimization
- Feeding time of Zn
- Mechanism
- argE gene