TY - JOUR
T1 - Green and sustainable FAME production
T2 - Enhanced continuous catalysis based on biofilm surface-displayed enzymes
AU - Ren, Peifang
AU - Zhao, Wei
AU - Zhou, Chaowei
AU - Chen, Tianpeng
AU - Sun, Wenjun
AU - Chen, Yong
N1 - Publisher Copyright:
© 2025 Elsevier B.V.
PY - 2025/6
Y1 - 2025/6
N2 - Fatty acid methyl esters (FAME) are the primary constituents of biodiesel, known for their renewability and environmental benefits. However, during the production of FAME, particularly when homogeneous catalysts are employed, the incomplete separation of the catalyst from the reaction medium poses a significant technical challenge. In this study, we developed an editable, sustainable, and efficient living material for FAME production based on a biofilm system. By overexpressing TasA in Bacillus subtilis we enhanced biofilm formation and created a functional carrier material with lipase activity. The SpyTag/SpyCatcher system immobilizes LipA onto the biofilm surface, improving enzyme stability and reusability. The resulting biofilm displayed a high catalytic capacity for FAME synthesis and could be regenerated to maintain enzyme activity. The living material could catalyze six consecutive batches with a maximum yield of 0.962 nmol mL−1. This approach offers a green and sustainable solution for industrial biocatalysis, aligning with environmental sustainability goals.
AB - Fatty acid methyl esters (FAME) are the primary constituents of biodiesel, known for their renewability and environmental benefits. However, during the production of FAME, particularly when homogeneous catalysts are employed, the incomplete separation of the catalyst from the reaction medium poses a significant technical challenge. In this study, we developed an editable, sustainable, and efficient living material for FAME production based on a biofilm system. By overexpressing TasA in Bacillus subtilis we enhanced biofilm formation and created a functional carrier material with lipase activity. The SpyTag/SpyCatcher system immobilizes LipA onto the biofilm surface, improving enzyme stability and reusability. The resulting biofilm displayed a high catalytic capacity for FAME synthesis and could be regenerated to maintain enzyme activity. The living material could catalyze six consecutive batches with a maximum yield of 0.962 nmol mL−1. This approach offers a green and sustainable solution for industrial biocatalysis, aligning with environmental sustainability goals.
KW - Amyloid fiber
KW - Biofilm surface display
KW - Enzymatic production
KW - Fatty acid methyl ester
UR - http://www.scopus.com/inward/record.url?scp=85219318730&partnerID=8YFLogxK
U2 - 10.1016/j.algal.2025.103984
DO - 10.1016/j.algal.2025.103984
M3 - 文章
AN - SCOPUS:85219318730
SN - 2211-9264
VL - 88
JO - Algal Research
JF - Algal Research
M1 - 103984
ER -