HPLC-CAD 法同时测定烟用浸膏类香原料中10 种水溶性糖

To determine the water-soluble sugar contents in tobacco flavor concretes, an HPLC-CAD method was established and optimized. Through systematic optimization of chromatographic conditions, CAD parameters, and sample extraction conditions, effective separation and accurate quantification of ten water-soluble sugars, including fructose, arabinose, rhamnose, galactose, mannose, xylose, maltose, glucose, sucrose, and sorbitol, were achieved. The results showed that: 1) The overall extraction efficiency was better when water was used as the solvent instead of a 50% aqueous acetonitrile solution. 2) The HPLC setup using a gradient elution with a mixture of 0.4% triethylamine in water and acetonitrile, at a flow rate of 0.6 mg/min, while maintaining the column temperature at 40 ℃, coupled with CAD parameters of a collection frequency of 5 Hz, a detector chamber temperature of 50 ℃, and a peak width setting of 0.02, provided optimal separation of the target analytes. 3) Under the optimized conditions, the linear regression equations for the ten sugars had better correlation coefficients (R² > 0.999) in the range of 0.05-1.00 mg/mL, the limits of detection and limits of quantification were in the range of 0.33-0.60 and 1.11-1.99 mg/g, respectively. The recoveries of the analytes ranged from 80.81% to 104.76%, with intra- and inter-day RSDs ranging from 1.03% to 5.17% and 4.42% to 6.97%, respectively. 4) The method was applied to a survey of 45 commonly used tobacco flavor concrete samples, and the results showed that glucose, fructose, maltose, sucrose, and sorbitol were the five sugar compounds with higher detection rates. The results of this method are accurate and reliable, and it is suitable for the determination of water-soluble sugars in tobacco flavor concretes.