Identification and characterization of a PTA-degrading bacterial strain

Yangyang Feng; Jun Chen; Bo Liu; Yingwen Chen; Guoyang Zheng; Jianhua Lu; Shubao Shen

Identification and characterization of a PTA-degrading bacterial strain

Yangyang Feng, Jun Chen, Bo Liu, Yingwen Chen, Guoyang Zheng, Jianhua Lu, Shubao Shen

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company. The 16SrDNA fragment was amplified from strain PA-18 by PCR, and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp. In the phylogenetic framework of bacterial classification, PA-18 belongs to Pseudomonas sp. Under the condition of temperature 37°C, pH 7.0 and OD_660nm 2.0, the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h. Meanwhile, this strain also could treat PTA wastewater with COD_Cr removal of 85%. p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.

Original language	English
Pages (from-to)	1968-1973
Number of pages	6
Journal	Huagong Xuebao/CIESC Journal
Volume	57
Issue number	8
State	Published - Aug 2006

Keywords

16SrDNA
Identification
Pseudomonas sp.
p-phthalic acid

Cite this

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title = "Identification and characterization of a PTA-degrading bacterial strain",

abstract = "A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company. The 16SrDNA fragment was amplified from strain PA-18 by PCR, and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp. In the phylogenetic framework of bacterial classification, PA-18 belongs to Pseudomonas sp. Under the condition of temperature 37°C, pH 7.0 and OD660nm 2.0, the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h. Meanwhile, this strain also could treat PTA wastewater with CODCr removal of 85%. p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.",

keywords = "16SrDNA, Identification, Pseudomonas sp., p-phthalic acid",

author = "Yangyang Feng and Jun Chen and Bo Liu and Yingwen Chen and Guoyang Zheng and Jianhua Lu and Shubao Shen",

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language = "英语",

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pages = "1968--1973",

journal = "Huagong Xuebao/CIESC Journal",

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TY - JOUR

T1 - Identification and characterization of a PTA-degrading bacterial strain

AU - Feng, Yangyang

AU - Chen, Jun

AU - Liu, Bo

AU - Chen, Yingwen

AU - Zheng, Guoyang

AU - Lu, Jianhua

AU - Shen, Shubao

PY - 2006/8

Y1 - 2006/8

N2 - A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company. The 16SrDNA fragment was amplified from strain PA-18 by PCR, and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp. In the phylogenetic framework of bacterial classification, PA-18 belongs to Pseudomonas sp. Under the condition of temperature 37°C, pH 7.0 and OD660nm 2.0, the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h. Meanwhile, this strain also could treat PTA wastewater with CODCr removal of 85%. p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.

AB - A strain PA-18 was isolated from the active sludge of p-phthalic acid (PTA) wastewater treatment in Yangtze Petrochemical Company. The 16SrDNA fragment was amplified from strain PA-18 by PCR, and the 16SrDNA sequence analysis indicated that strain PA-18 shared 99% 16SrDNA sequence homology with Pseudomonas sp. In the phylogenetic framework of bacterial classification, PA-18 belongs to Pseudomonas sp. Under the condition of temperature 37°C, pH 7.0 and OD660nm 2.0, the PTA decomposition rate of this bacterial strain was beyond 95% after 24 h. Meanwhile, this strain also could treat PTA wastewater with CODCr removal of 85%. p-Phthalic acid was degraded by the strain PA-18 through the intermediate formation of protocatechuic acid in the aerobic state.

KW - 16SrDNA

KW - Identification

KW - Pseudomonas sp.

KW - p-phthalic acid

UR - http://www.scopus.com/inward/record.url?scp=33750029708&partnerID=8YFLogxK

M3 - 文章

AN - SCOPUS:33750029708

SN - 0438-1157

VL - 57

SP - 1968

EP - 1973

JO - Huagong Xuebao/CIESC Journal

JF - Huagong Xuebao/CIESC Journal

IS - 8

ER -

Identification and characterization of a PTA-degrading bacterial strain

Abstract

Keywords

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