Identification of lignocellulosic derivatives inhibiting succinic acid fermentation and molecular mechanism investigation

Chao Xu; Md Asraful Alam; Jun Zhang; Zhongming Wang; Huanjun Chen; Chunliang Xie; Yuande Peng; Shushi Huang; Wei Zhuang; Jingliang Xu

doi:10.1016/j.indcrop.2022.115466

Identification of lignocellulosic derivatives inhibiting succinic acid fermentation and molecular mechanism investigation

Chao Xu, Md Asraful Alam, Jun Zhang, Zhongming Wang, Huanjun Chen, Chunliang Xie, Yuande Peng, Shushi Huang, Wei Zhuang, Jingliang Xu

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Phenolic derivatives from lignocellulosic biomass under harsh pretreatment conditions may inhibit microorganisms. Waste liquid produced from alkali pretreatment of sugarcane bagasse (SCB) was qualitatively and quantitatively analyzed. Model compounds were used to evaluate the effects of major derivatives of alkali pretreatment liquid (APL) on the growth and succinic acid (SA) synthesis of Actinobacillus succinogenes ATCC 55618, and molecular mechanisms were analyzed with transcriptome and proteome sequencing. The 10 most abundant measured components in APL were all lignin degradation products, and they showed different degrees of inhibitory effects on A. succinogenes. Benzoic acid and p-hydroxy benzaldehyde exhibited most serious inhibition to A. succinogenes, causing biomass and SA concentration to decrease by over 30 % and 45 %, respectively. This could be attributed to the presence of inhibitors caused the down-regulation of genes including phosphofructokinase, xylulose kinase, pyruvate carboxylase, and acetate kinase, which are crucial in pathways for sugar uptake, CO₂ fixation, and reducing power supply.

Original language	English
Article number	115466
Journal	Industrial Crops and Products
Volume	187
DOIs	https://doi.org/10.1016/j.indcrop.2022.115466
State	Published - 1 Nov 2022

Keywords

Derivatives
Inhibitory effect
Lignocellulosic biomass
Metabolism
Succinic acid

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10.1016/j.indcrop.2022.115466

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title = "Identification of lignocellulosic derivatives inhibiting succinic acid fermentation and molecular mechanism investigation",

abstract = "Phenolic derivatives from lignocellulosic biomass under harsh pretreatment conditions may inhibit microorganisms. Waste liquid produced from alkali pretreatment of sugarcane bagasse (SCB) was qualitatively and quantitatively analyzed. Model compounds were used to evaluate the effects of major derivatives of alkali pretreatment liquid (APL) on the growth and succinic acid (SA) synthesis of Actinobacillus succinogenes ATCC 55618, and molecular mechanisms were analyzed with transcriptome and proteome sequencing. The 10 most abundant measured components in APL were all lignin degradation products, and they showed different degrees of inhibitory effects on A. succinogenes. Benzoic acid and p-hydroxy benzaldehyde exhibited most serious inhibition to A. succinogenes, causing biomass and SA concentration to decrease by over 30 % and 45 %, respectively. This could be attributed to the presence of inhibitors caused the down-regulation of genes including phosphofructokinase, xylulose kinase, pyruvate carboxylase, and acetate kinase, which are crucial in pathways for sugar uptake, CO2 fixation, and reducing power supply.",

keywords = "Derivatives, Inhibitory effect, Lignocellulosic biomass, Metabolism, Succinic acid",

author = "Chao Xu and Alam, {Md Asraful} and Jun Zhang and Zhongming Wang and Huanjun Chen and Chunliang Xie and Yuande Peng and Shushi Huang and Wei Zhuang and Jingliang Xu",

note = "Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = nov,

day = "1",

doi = "10.1016/j.indcrop.2022.115466",

language = "英语",

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TY - JOUR

T1 - Identification of lignocellulosic derivatives inhibiting succinic acid fermentation and molecular mechanism investigation

AU - Xu, Chao

AU - Alam, Md Asraful

AU - Zhang, Jun

AU - Wang, Zhongming

AU - Chen, Huanjun

AU - Xie, Chunliang

AU - Peng, Yuande

AU - Huang, Shushi

AU - Zhuang, Wei

AU - Xu, Jingliang

PY - 2022/11/1

Y1 - 2022/11/1

N2 - Phenolic derivatives from lignocellulosic biomass under harsh pretreatment conditions may inhibit microorganisms. Waste liquid produced from alkali pretreatment of sugarcane bagasse (SCB) was qualitatively and quantitatively analyzed. Model compounds were used to evaluate the effects of major derivatives of alkali pretreatment liquid (APL) on the growth and succinic acid (SA) synthesis of Actinobacillus succinogenes ATCC 55618, and molecular mechanisms were analyzed with transcriptome and proteome sequencing. The 10 most abundant measured components in APL were all lignin degradation products, and they showed different degrees of inhibitory effects on A. succinogenes. Benzoic acid and p-hydroxy benzaldehyde exhibited most serious inhibition to A. succinogenes, causing biomass and SA concentration to decrease by over 30 % and 45 %, respectively. This could be attributed to the presence of inhibitors caused the down-regulation of genes including phosphofructokinase, xylulose kinase, pyruvate carboxylase, and acetate kinase, which are crucial in pathways for sugar uptake, CO2 fixation, and reducing power supply.

AB - Phenolic derivatives from lignocellulosic biomass under harsh pretreatment conditions may inhibit microorganisms. Waste liquid produced from alkali pretreatment of sugarcane bagasse (SCB) was qualitatively and quantitatively analyzed. Model compounds were used to evaluate the effects of major derivatives of alkali pretreatment liquid (APL) on the growth and succinic acid (SA) synthesis of Actinobacillus succinogenes ATCC 55618, and molecular mechanisms were analyzed with transcriptome and proteome sequencing. The 10 most abundant measured components in APL were all lignin degradation products, and they showed different degrees of inhibitory effects on A. succinogenes. Benzoic acid and p-hydroxy benzaldehyde exhibited most serious inhibition to A. succinogenes, causing biomass and SA concentration to decrease by over 30 % and 45 %, respectively. This could be attributed to the presence of inhibitors caused the down-regulation of genes including phosphofructokinase, xylulose kinase, pyruvate carboxylase, and acetate kinase, which are crucial in pathways for sugar uptake, CO2 fixation, and reducing power supply.

KW - Derivatives

KW - Inhibitory effect

KW - Lignocellulosic biomass

KW - Metabolism

KW - Succinic acid

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U2 - 10.1016/j.indcrop.2022.115466

DO - 10.1016/j.indcrop.2022.115466

M3 - 文章

AN - SCOPUS:85135841501

SN - 0926-6690

VL - 187

JO - Industrial Crops and Products

JF - Industrial Crops and Products

M1 - 115466

ER -

Identification of lignocellulosic derivatives inhibiting succinic acid fermentation and molecular mechanism investigation

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Keywords

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