Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles

Honghua Jia, Fan Huang, Zhen Gao, Chao Zhong, Hua Zhou, Min Jiang, Ping Wei

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe3O4 nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe3O4 nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe3O4 nanoparticles was located between 30-40 nm ω-TA was immobilized onto magnetic PVA-Fe3O4 nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6-8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalBiotechnology Reports
Volume10
DOIs
StatePublished - 1 Jun 2016

Keywords

  • Chiral amine
  • Immobilization
  • Magnetic PVA-FeO nanoparticles
  • ω-transaminase

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