Abstract
ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe3O4 nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe3O4 nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe3O4 nanoparticles was located between 30-40 nm ω-TA was immobilized onto magnetic PVA-Fe3O4 nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6-8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes.
Original language | English |
---|---|
Pages (from-to) | 49-55 |
Number of pages | 7 |
Journal | Biotechnology Reports |
Volume | 10 |
DOIs | |
State | Published - 1 Jun 2016 |
Keywords
- Chiral amine
- Immobilization
- Magnetic PVA-FeO nanoparticles
- ω-transaminase