TY - JOUR
T1 - Immobilization of Burkholderia cepacia lipase on functionalized ionic liquids modified mesoporous silica SBA-15
AU - Tang, Susu
AU - Hu, Yi
AU - Yu, Dinghua
AU - Zou, Bin
AU - Jiang, Ling
PY - 2012/9
Y1 - 2012/9
N2 - Mesoporous silica SBA-15 was modified by imidazole-based ionic liquids with alkyl group. The modified support samples were characterized by nitrogen adsorption-desorption, small-angle X-ray diffraction, Fourier transform infrared spectroscopy, elemental analysis, and scanning electron microscopy. The samples were used to immobilize Burkholderia cepacia lipase (BCL) and the influence of alkyl chain length of ionic liquid on enzymatic properties was investigated by the hydrolysis reaction of triacetin. The results revealed that functionalized ionic liquid modification did not destroy the structure of SBA-15. Compared with the immobilized lipase on parent SBA-15, the immobilized lipases on modified support samples were less sensitive to temperature and low pH and showed higher specific activity and stability. Thereinto, BCL immobilized on methyl-functionalized ionic liquid modified SBA-15 had the highest specific activity, which improved 2.4 folds compared with BCL immobilized on SBA-15. BCL immobilized on octyl-functionalized ionic liquid modified SBA-15 had the best thermal stability, reusability, storage stability, and stability in organic solvent.
AB - Mesoporous silica SBA-15 was modified by imidazole-based ionic liquids with alkyl group. The modified support samples were characterized by nitrogen adsorption-desorption, small-angle X-ray diffraction, Fourier transform infrared spectroscopy, elemental analysis, and scanning electron microscopy. The samples were used to immobilize Burkholderia cepacia lipase (BCL) and the influence of alkyl chain length of ionic liquid on enzymatic properties was investigated by the hydrolysis reaction of triacetin. The results revealed that functionalized ionic liquid modification did not destroy the structure of SBA-15. Compared with the immobilized lipase on parent SBA-15, the immobilized lipases on modified support samples were less sensitive to temperature and low pH and showed higher specific activity and stability. Thereinto, BCL immobilized on methyl-functionalized ionic liquid modified SBA-15 had the highest specific activity, which improved 2.4 folds compared with BCL immobilized on SBA-15. BCL immobilized on octyl-functionalized ionic liquid modified SBA-15 had the best thermal stability, reusability, storage stability, and stability in organic solvent.
KW - Burkholderia cepacia lipase
KW - Immobilization
KW - Ionic liquid
KW - Mesoporous silica
KW - Modification
UR - http://www.scopus.com/inward/record.url?scp=84867946792&partnerID=8YFLogxK
U2 - 10.3724/SP.J.1088.2012.20457
DO - 10.3724/SP.J.1088.2012.20457
M3 - 文章
AN - SCOPUS:84867946792
SN - 1872-2067
VL - 33
SP - 1565
EP - 1571
JO - Chinese Journal of Catalysis
JF - Chinese Journal of Catalysis
IS - 9
ER -