Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)

Changqing Yang; Tao Zheng; Chenchen Yu; Jun Zhou; Bingfang He; Xiaoning Liu

doi:10.3969/j.issn.0438-1157.2014.05.036

Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)

Changqing Yang, Tao Zheng, Chenchen Yu, Jun Zhou, Bingfang He, Xiaoning Liu

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

Abstract

In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was, the higher the activity of immobilized lipase was.

Original language	English
Pages (from-to)	1815-1820
Number of pages	6
Journal	Huagong Xuebao/CIESC Journal
Volume	65
Issue number	5
DOIs	https://doi.org/10.3969/j.issn.0438-1157.2014.05.036
State	Published - May 2014

Keywords

Atom transfer radical polymerization
Biocatalysis
Flexible immobilization of enzyme
Length of chain
Organic solvent-tolerant lipase
Polymers
Tentacles-like support with chain containing epoxy group

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10.3969/j.issn.0438-1157.2014.05.036

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title = "Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)",

abstract = "In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was, the higher the activity of immobilized lipase was.",

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author = "Changqing Yang and Tao Zheng and Chenchen Yu and Jun Zhou and Bingfang He and Xiaoning Liu",

year = "2014",

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doi = "10.3969/j.issn.0438-1157.2014.05.036",

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AU - Yang, Changqing

AU - Zheng, Tao

AU - Yu, Chenchen

AU - Zhou, Jun

AU - He, Bingfang

AU - Liu, Xiaoning

PY - 2014/5

Y1 - 2014/5

N2 - In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was, the higher the activity of immobilized lipase was.

AB - In order to improve the enzyme conformation for the negative effect of the rigid immobilized enzyme, the hydrophilic and flexible support PS-acyl-P(acrylamide-co-glycidyl methacrylate) with tentacles-like chain containing epoxy group was prepared with the atom transfer radical polymerization (ATRP) method. A series of chain length of tentacles-like support with epoxy group were prepared via changing the proportion of the total monomers to the initiator, and were used for flexible immobilization of organic solvent-tolerant lipase (YCJ01) covalently. The major objective of this paper was to investigate the effect of different lengthes of chain on the activity of immobilized lipase. The longer the chain length (weight gain <3200%) was, the higher the activity of immobilized lipase was.

KW - Atom transfer radical polymerization

KW - Biocatalysis

KW - Flexible immobilization of enzyme

KW - Length of chain

KW - Organic solvent-tolerant lipase

KW - Polymers

KW - Tentacles-like support with chain containing epoxy group

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JO - Huagong Xuebao/CIESC Journal

JF - Huagong Xuebao/CIESC Journal

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ER -

Influence of tentacles-like support with chain containing epoxy group on activity of immobilized lipase (YCJ01)

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Keywords

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